Therapeutic role of stem cells versus curcumin nanoemulsion on cadmium induced acute testicular damage in adult albino rats : Histological, immunohistochemical and biochemical study / Hend Ahmed Abdallah Badawi ; Supervised Hany Abdellatif Soliman , Shereen Abdelfattah Mahamed , Rasha Abdelkhalek Attia Radwan

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Anatomy

Cadmium (Cd), a grave occupational pollutant, can result in; testicular damage. This study was designed to distinguish the potential effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) versus that of curcumin nanoemulsion on Cdinduced testicular damage. Fifty adult male Sprague Dawley rats were distributed into five groups; control, sham control, Cd-treated, stem cell-treated and nanocurcumintreated groups. Histological, immune histochemical; caspase 3 and proliferating cell nuclear antigen (PCNA),testosterone levels, nitric oxide, malondialdehyde (MDA)/glutathione (GSH) superoxide, dismutase (SOD), Western blot; B-cell lymphoma (Bcl-2), BCL2-Associated X Protein (BAX), BAX/Bcl-2 ratio and morphometry were done. Cadmium-treated group showed degenerated, detached seminiferous tubules, vacuolations and wide interstitial spaces containing fluid exudates.The same group revealed increased expression of BAX, BAX/Bcl-2 ratio, caspase 3and increased mean values of MDA, NO. Concomitantly, Cd has significant reduction in PCNA, Bcl-2 and sperm cell count when compared to control group. BM-MSCs- and nanocurcumin-treated groups revealed well-structured tubules and were perceived to expressively enhance the deleterious changes induced by Cd. The injurious changes on the testis induced by Cd were obviously improved when treated with either MSCs or nano-curcumin. BM-MSCs exerted more ameliorative changes

Issued also as CD