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Genotyping of -31T/C and -511C/T polymorphism in the interleukin 1Beta (IL1B) promoter in keratoconus patients / Aliaa Abedelfattha Moahmed Katamech ; Supervised Ismail A. Abdelhamid , Somaia M. Ismail

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Aliaa Abdelfatth Moahmed Katmech , 2020Description: 66 P. : charts , facimiles ; 25cmOther title:
  • التنميط الوراثى لكل من التعدد الشكلى (تى / سى 31) - و (سى / تى511) فى بداءة الانتلرليوكن بيتا 1 (آى ال آى بى ) فى مرضى القرنية المخروطية [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology Summary: Purpose: Study the association of IL1B promoter polymorphisms rs1143627 ({u2212}31 T>C) and rs16944 ({u2212}511 C>T) and the risk of keratoconus in Egyptian population, establishing a biomarker for managing of keratoconus cases and in order to give convenient genetic counseling. Methods: The association between -31T/C and -511C/T polymorphism in the interleukin 1beta (IL1B) promoter and Egyptian keratoconus patients were investigated.This study included 159 blood samples that belong to unrelated affected cases and 153 healthy individuals for rs16944 ({u2212}511 C>T) polymorphism, 144blood samples that belong to unrelated affected cases and 141healthy individuals forrs1143627 ({u2212}31 T>C) polymorphism. All cases had been examined in the Research Institute of ophthalmology and written informed consents were obtained from all participating individuals. Polymerase chain reaction (PCR) {u2013} restriction fragment length polymorphism (RFLP) analysis were used for screening of rs1143627 ({u2212}31 T>C) and rs16944 ({u2212}511 C>T) polymorphisms. Results: The {u2212}511 (rs16944) and {u2212}31 (rs1143627) polymorphismsin the promoter region of IL1B have been analyzed. The C/C genotype frequency of rs1143627 ({u2212}31T>C p=0.001,)had a statisticallysignificant association with increasing risk ofkeratoconus. The C/C genotype frequency of rs16944({u2212}511C>T p=0.066,) was not statistically associated with increasing risk of keratoconus. However the TT genotype is more frequent in patients than controls (22.6% vs7.5%
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2020.Al.G (Browse shelf(Opens below)) Not for loan 01010110082311000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.12.25.M.Sc.2020.Al.G (Browse shelf(Opens below)) 82311.CD Not for loan 01020110082311000

On title page the auther Aliaa Abedelfattha Moahmed Katamech

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology

Purpose: Study the association of IL1B promoter polymorphisms rs1143627 ({u2212}31 T>C) and rs16944 ({u2212}511 C>T) and the risk of keratoconus in Egyptian population, establishing a biomarker for managing of keratoconus cases and in order to give convenient genetic counseling. Methods: The association between -31T/C and -511C/T polymorphism in the interleukin 1beta (IL1B) promoter and Egyptian keratoconus patients were investigated.This study included 159 blood samples that belong to unrelated affected cases and 153 healthy individuals for rs16944 ({u2212}511 C>T) polymorphism, 144blood samples that belong to unrelated affected cases and 141healthy individuals forrs1143627 ({u2212}31 T>C) polymorphism. All cases had been examined in the Research Institute of ophthalmology and written informed consents were obtained from all participating individuals. Polymerase chain reaction (PCR) {u2013} restriction fragment length polymorphism (RFLP) analysis were used for screening of rs1143627 ({u2212}31 T>C) and rs16944 ({u2212}511 C>T) polymorphisms. Results: The {u2212}511 (rs16944) and {u2212}31 (rs1143627) polymorphismsin the promoter region of IL1B have been analyzed. The C/C genotype frequency of rs1143627 ({u2212}31T>C p=0.001,)had a statisticallysignificant association with increasing risk ofkeratoconus. The C/C genotype frequency of rs16944({u2212}511C>T p=0.066,) was not statistically associated with increasing risk of keratoconus. However the TT genotype is more frequent in patients than controls (22.6% vs7.5%

Issued also as CD

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