Evaluation of moringa oleifera plant against hepato- nephrotoxicity induced by lead acetate in rabbits / Nancy Badawi Mohamed Hassan ; Supervised Amira H. Mohamed , Nashwa Adel Abuaita , Somia Ayesh Nassar

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Clinical Pathology

Two experiments were conducted to evaluate the effect of Moringa oleifera leaf ethanol extract (MOLEE) as adjunct and prophylactic treatments of lead acetate-induced hepato-nephrotoxicity in rabbits. In the first experiment, 36 male New Zealand White rabbits were assigned into two groups. The first group (14 rabbits) served as normal control. The second group (22 rabbits) was administered orally lead acetate at a concentration of 40 mg/kg/day, 5 days/week for 8 weeks. At the 4th and the 8th week of treatment, 6 animals (3 animals at each period) of the second group were sacrificed while the remaining animals (16 rabbits) were assigned randomly into 2 subgroups (8 rabbits each): treated and non-treated. The first subgroup was orally given 1 mL phosphate-buffered saline for further 4 weeks while the second subgroup was administered orally (MOLEE) at a dose of 400 mg/kg/day for the same period. Blood samples were collected to determine hematological and serum biochemical parameters. Tissue specimens were collected from the liver and kidney for evaluation of the oxidant/antioxidant markers and for histopathological examination. Lead acetate exposure decreased the mean body weight gain, hematocrit, mean corpuscular volume, and lymphocytes{u2019} count. Moreover, it markedly increased counts of monocytes and platelets, serumenzymeactivity levelsofcreatinine, totalcholesterol, triglycerides, and low-density lipoprotein cholesterol

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