Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells / Marianne Edward Gad Elkareem Yassa ; Supervised Iman Ahmed Maher Mansour , Tagrid Gaafar , Nadia Ibrahim Sewelam

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Marianne Edward Gad Elkareem Yassa

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TextLanguage: English Publication details: Cairo : Marianne Edward Gad Elkareem Yassa , 2016Description: 220 P. : facsimiles ; 25cmOther title:

تمايز الخلايا الجزعيه الانسانيه المحفزه لتكون متعدده القدرات الي خلايا عضله القلب [Added title page title]

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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology Summary: Cardiac diseases are among the main causes of morbidity and mortality. Despite significant advances in medical and interventional therapy, cell replacement is considered as a promising therapeutic modality for cardiac diseases. hiPSCs offer the potential to generate large numbers of functional cardiomyocytes from patient-specific cell sources bypassing ethicalissues and the immunological rejection ofother stem cells sources. The most successful differentiation approaches are those recapitulatingthe regulatory pathways that control the establishment of the corresponding lineage in the early embryo.The aim of the study was to differentiate NP0040 hiPSC line into cardiomyocytes andto show evidence of cardiac differentiation. Cardiac differentiation was done by temporally modulating the regulatory elements of the signaling pathways: wnt, BMP-4, FGF&ascorbic acid in a monolayer-based culture system under serum-free, feeder-free conditions with subsequent purification by 2Lactate method3. Characterization of the generated cardiomyocytes was done by flow cytometry, immunofluorescence,reverse-transcriptase PCR and electrophysiological studies.Here we showed thattemporal modulation of wnt signaling is essential for mesoderm induction and cardiac specification (protocol (A)

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Item type	Current library	Home library	Call number	Copy number	Status	Date due	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.11.07.Ph.D.2016.Ma.C (Browse shelf(Opens below))		Not for loan		01010110069388000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.11.07.Ph.D.2016.Ma.C (Browse shelf(Opens below))	69388.CD	Not for loan		01020110069388000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology

Cardiac diseases are among the main causes of morbidity and mortality. Despite significant advances in medical and interventional therapy, cell replacement is considered as a promising therapeutic modality for cardiac diseases. hiPSCs offer the potential to generate large numbers of functional cardiomyocytes from patient-specific cell sources bypassing ethicalissues and the immunological rejection ofother stem cells sources. The most successful differentiation approaches are those recapitulatingthe regulatory pathways that control the establishment of the corresponding lineage in the early embryo.The aim of the study was to differentiate NP0040 hiPSC line into cardiomyocytes andto show evidence of cardiac differentiation. Cardiac differentiation was done by temporally modulating the regulatory elements of the signaling pathways: wnt, BMP-4, FGF&ascorbic acid in a monolayer-based culture system under serum-free, feeder-free conditions with subsequent purification by 2Lactate method3. Characterization of the generated cardiomyocytes was done by flow cytometry, immunofluorescence,reverse-transcriptase PCR and electrophysiological studies.Here we showed thattemporal modulation of wnt signaling is essential for mesoderm induction and cardiac specification (protocol (A)

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