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Development of nano-based biosensor for FMDV / Mervat Hamdy El-Sayed ; Supervised Hussein Aly Hussein Ahmed , Ayman Hany Mahmoud Metwally Eldeeb , Taher Salah Eldin Ahmed

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Mervat Hamdy Elsayed , 2016Description: 98 P. : charts , facsimiles ; 25cmOther title:
  • استنباط مجس استشعار مبني على النانو لفيروس مرض الحمى القلاعية [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology Summary: Nano-PCR represents a recent sensitive diagnostic tool for viral diseases depending on the fundamental Nano effects of AuNPs. In the present work, AuNPs- FMDV biosensor is designed with thiol-linked oligonucleotides for the conserved 3D gene of FMDV. This biosensor has been validated with RNA standard and 10 swine vesicular disease virus isolates (SVDV) as a negative control. Likewise, it has been applied on 13 viral isolates (IZSLER institute) and 31 clinical samples collected from Egypt during March 2012 to September 2015. AuNPs-FMDV biosensor demonstrates superior analytical and clinical performance for FMDV diagnosis. The analytical sensitivity, dynamic range and limit of detection (LOD) of AuNPs {u2013} FMDV biosensor was 100 copies number RNA standard in RT-PCR and 1 copy number RNA standard in real time RT-PCR (rRT-PCR) with 94.5% efficiency, 0.989 R2, -3.544 Slope and 100% specificity without cross-reactivity with SVDV. It has a simple workflow, and it accelerates the epidemiological surveillance. Hence, it is suitable for quarantine stations and farms for diagnosis, particularly in FMDV endemic areas
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Item type Current library Home library Call number Copy number Status Date due Barcode
Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2016.Me.D (Browse shelf(Opens below)) Not for loan 01010110072527000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.10.17.M.Sc.2016.Me.D (Browse shelf(Opens below)) 72527.CD Not for loan 01020110072527000

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology

Nano-PCR represents a recent sensitive diagnostic tool for viral diseases depending on the fundamental Nano effects of AuNPs. In the present work, AuNPs- FMDV biosensor is designed with thiol-linked oligonucleotides for the conserved 3D gene of FMDV. This biosensor has been validated with RNA standard and 10 swine vesicular disease virus isolates (SVDV) as a negative control. Likewise, it has been applied on 13 viral isolates (IZSLER institute) and 31 clinical samples collected from Egypt during March 2012 to September 2015. AuNPs-FMDV biosensor demonstrates superior analytical and clinical performance for FMDV diagnosis. The analytical sensitivity, dynamic range and limit of detection (LOD) of AuNPs {u2013} FMDV biosensor was 100 copies number RNA standard in RT-PCR and 1 copy number RNA standard in real time RT-PCR (rRT-PCR) with 94.5% efficiency, 0.989 R2, -3.544 Slope and 100% specificity without cross-reactivity with SVDV. It has a simple workflow, and it accelerates the epidemiological surveillance. Hence, it is suitable for quarantine stations and farms for diagnosis, particularly in FMDV endemic areas

Issued also as CD

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