Some studies on eimeria tenella infecting chicken / Amira Mohamed Ibrahim ; Supervised Waheed Mohamed Ali Mosa , Nisreen Ezz Eldien Mahmoud , Amal Ibrahim Elkhawaga

Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Parasitology

Our study was directed to collect caecal samples from 600 broiler chicken during the period from January to December 2015 from Cairo and Giza chicken farms. The caeca were scraped for Eimeria tenella oocysts collection which were propagated in 49 one day old chicks. Weekly antibody titre was measured in the sera of infected chicks using Enzyme-linked immunosorbent assay and Agar Gel Precipitation Test and these serological tests revealed the presence of antibody against Eimeria tenella which was found in 68% of the examined samples. Maternal immunity was notice in the 1st week of age and decreased till reaching the 4th week in our study. The propagated oocysts were identified morphlologically using micrometer and molecularly using Polymerase Chain Reaction. The molecular identification of Eimeria tenella was targeting the Internal Transcribed Spacer 1(ITS-1) region and the amplified fragment consists of 278 bp. the phylogenetic analysis was also done and revealed 99.6% identity with other global isolates on the Genbank. Transmission electron microscopy used to detect the ultrastructural components of Eimeria tenella endogenous stages in caecal tissue. Immunohistochemical study was conducted on the infected caecal tissue and revealed a positive reaction Histopathological examination was conducted on the infected caecal tissue and revealed that Eimeria tenella infection

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