Chemical and biological investigation of Ferula tingitana L. growing in Libya / Waleed Abourawi Alghwaji ; Supervised Aly M. Elsayed , Kadriya Shafik Eldeeb , Abeer M. Elsayed

Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy

To contribute new knowledge to the currently existing known chemical/or biological data about Ferula tingitana L. growing in Libya. GC/MS of the lipoidal profile was investigated. Daucol is the major recognized sesquiterpene. Linolenic acid is the main makeup of fatty acids. The chemical composition of the essential oil of flower (0.06% w/v) and leaves (0.1% w/v) of F. tingitana was comparatively analyzed by GC-MS. Furthermore, to throw light on antimicrobial and cytotoxic activities of oils derived Libyan plant. The main constituents of both flower-and leaves-derived oil samples were Ü-thujene (13.5-2.3%), elemol (8.9-8.3%), eudesmol (0.6-9.7%) and cadinol (2.2-13.8%) respectively. The principle difference was a considerably more pronounced sesquiterpenes presence in the leaves-oil, amounting to 74.0%, than in the flower counterpart (39.9%). Chromatographic analysis of the soluble fractions of the ethanolic extract were afforded ?-sitosterol 1, colladonin 2, scopoletin 3, caffeic acid 4, sesquiterpenecoumarin 5 and 1-(3,4-dihydroxycinnamoyl)cyclopentane-2`,3`-diol 6. Leaves-oil sample being mostly efficient as antibacterial against Bacillus subtilis and Neisseria gonorrhea with potency 48.3, 41.9% compared to tetracycline standard antibacterial drug. The essential oil samples revealed marked in vitro cytotoxicity against breast (MCF-7), cervical (HELA) and liver (HEPG2) carcinoma cell lines with IC50% (6.9, 4.8), (8.6, 10.9) and (4.4, 4.2) for the flower-,leaves- derived oil sample, respectively

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