Enhancement of the regenerative capabilities of the neuro- ectomised dog peripheral nerves using nano techniques / Warda Mosbah Abdelhafiz ; Supervised Mahmoud Ahmed Amer , Abdelwahab A. Elghareeb , Mohammad Ayad Abdelhameed

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology

Nano science is the science of small particles of materials on a nanometer scale in at least one dimension. Nanomaterials include nanotubes, nano fibers, liposomes, nanoparticles, polymeric micelles, nano gels and dendrimers. Our study aims at evaluating motor nerve regeneration using nanofibrils technique. Nine adult mongrel dogs were included in this study. A one and half cm gap was made in the nerve by direct cutting of both sides (proximal cut and then distal to avoid the double shock). The nanofibrils were used to fill the space and cover the two fixed ends in six of the dogs, another three animals were used as control, the nerve was cut and the space in between the two fixed ends was left without nanofibrils. Complete weight bearing occurred 2-3 weeks after implantation of the nanofibrils. All animals with implanted nanofibrils used their limb successfully at 3-4 weeks and the animal gait returned to its normal condition without any remarkable symptoms of lameness and the joints angles displayed its normal posture. Histopathological examination revealed better organized histological structures of nanofibre treated nerve samples than untreated control one with nearly complete bridging of nerve fibers across several bundles separated by thick perineural connective tissue. Thus we concluded that nanofibres can be regarded as an efficient regeneration enhancing material in peripheral nerve injuries

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