Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Dairy

This study was investigated to improve the functional properties of milk proteins by complexed or conjugated with some bioactive components including the psyllium husk mucilage (PHM), napeq mucilage (NapM) and caffeic acid (CA). Firstly, the recovery of milk proteins by non-covalent interaction with PHM and NapM were studied. Furthermore, syntheses the of Ý-lactoglobulin-CA (ÝLg-CA) conjugated at different pH (pH 2.5, 6.0, and 8.5) and its polymerization. The resulting bioconjugates were characterized by MALDI-TOF MS, differential scanning calorimetry (DSC), fluorescence-quenching, infrared, circular dichroism spectroscopies and SDS gels electrophoresis. Furthermore, applying some milk proteins-bioactive ingredients as the emulsifier and antioxidant in omega 3 oil in water emulsion, hepatoprotective rats fed high-fat diet and cytotoxicity agent against liver and breast cancer cells. Results showed: Recovered the buffalo milk proteins using PHM and NapM by two phases (TPS) and pH hydrostatic system. The % of milk protein recovery by TPS and pH system increased, which the BTMP/PHM complex (pH system) was 94.47 % compared to the traditional acid buffalo casein (BCas) and buffalo total milk proteins (BTMP prepared by pH system) as a control 77.10 and 85.52%, respectively

Issued also as CD