Oxidative DNA damage and modulation of p53 tumor suppressor gene as possible mechanisms of chromium carcinogenicity / Aisha Safwat Seif Eldin ; Supervised Nabil Abdallah Abdelmaksoud , Nermin Hamdy Mohammed , Fatma Mohammed Taha

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine -Toxicology

Subjects and methods: This work was carried out in twelve small-to medium-sized tanneries, in Misr Al Kadema district in Cairo. The study comprised of two groups, an exposed group (45 male workers) and a matched control group (30 male workers). All workers were subjected to a detailed history including present, past, family and occupational history. Clinical examination was performed with special emphasis on chest and skin examination. Laboratory investigations were performed in the form of kidney function test (blood urea and serum creatinine), blood level of TAC, P53 and chromium. Also, urinary 8-OHdG was done. Results: A significant increase in chromium, p53 and urinary 8-OHG in the exposed group was found with significant decrease of serum TAC compared to their referent control. Significant positive correlation between serum P53 with serum level of TAC. Conclusion: Results revealed that tannery workers have increased level of blood chromium, and this increase is associated with oxidative damage especially oxidative DNA damage reflected by decrease TAC serum level and elevated urinary 8-OHG. Also, up-regulation or activation of serum p53 in tannery workers which was positively correlated with chromium (non-significant) and significantly positively correlated with TAC reinforce the possible relation between oxidative stress induced by chromium exposure and carcinogenesis

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