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Purification, antiserum production, serological and molecular detection of Cucurbit Yellow Stunting Disorder Virus (CYSDV) in Egypt / Noura Hassan Mohamed ; Supervised Gamal Amin Mohamed Ghanem , Ahmed Ismail Abdelalim

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Noura Hassan Mohamed , 2016Description: 120 P. : facsimiles ; 25cmOther title:
  • تنقيــة وإنتـاج المصـل واختبـارات سيـرولوجيـة وجـزيئـيـة لفيروس إصفرار وتقزم القرعيات في مصر [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Plant Pathology and physiology Summary: Cucurbit yellow stunting disorder crinivirus (CYSDV) causes significant yield losses in field- and in greenhouse grown cucurbits in Egypt. Evalution of cucumber varietal reaction against CYSDV-infection using the induced antibody (CYSDV-Pab) in DAS-ELISA. The induced polyclonal antibody (CYSDV-Pab) was also evaluated in detecting CYSDV antigen in the infected plants through serological tests. Reverse transcription-polymerase chain reaction (RT-PCR) techniques was developed to detect CYSDV that target either the coat protein gene or p22. RT-LAMP was reliable for diagnosis of CYSDV-infected leaf samples and insect vectors from the field in 60 min
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.16.Ph.D.2016.No.P (Browse shelf(Opens below)) Not for loan 01010110070409000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.16.Ph.D.2016.No.P (Browse shelf(Opens below)) 70409.CD Not for loan 01020110070409000

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Plant Pathology and physiology

Cucurbit yellow stunting disorder crinivirus (CYSDV) causes significant yield losses in field- and in greenhouse grown cucurbits in Egypt. Evalution of cucumber varietal reaction against CYSDV-infection using the induced antibody (CYSDV-Pab) in DAS-ELISA. The induced polyclonal antibody (CYSDV-Pab) was also evaluated in detecting CYSDV antigen in the infected plants through serological tests. Reverse transcription-polymerase chain reaction (RT-PCR) techniques was developed to detect CYSDV that target either the coat protein gene or p22. RT-LAMP was reliable for diagnosis of CYSDV-infected leaf samples and insect vectors from the field in 60 min

Issued also as CD

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