Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Parasitology

Intestinal schistosomiasis is a serious health disease, caused by Schistosomamansoni. Biomphalariaalexandrinasnail is the intermediate host of S. mansoniin Egypt. Antigenic relationship between S.mansoniand B.alexandrinasnail both infected and noninfected with S.mansoni wasstudied.S.mansonicrude antigen was prepared from adult stage ofS.mansonicollected from experimentally infected mice. Crude antigens from both infected and non infectedB.alexandrina snail were also prepared after dissecting each animal snail into two parts foot and visceral hump. These antigens were fractionated using Sodium dodecyl sulfate Poly acrylamide Gel Electrophoresis (SDS PAGE). Common bands were detected among adult worm antigen( AWA) and both infected and non infectedB. alexandrina visceral hump antigens at 22,30 and 58 KDa , while a common band among AWA antigen and both infected and non infected foot antigens was detected at 40 KDa. Specific mice hyper immune serum (HIS)was prepared against each prepared antigen that was used in western blot.WB revealed that HIS of AWA is reactive to infected snailmore thannon infectedsnail. HIS of AWA identifieda common band in non infected snails both foot and visceral hump at 70 -75 KDaand identified a common band at 45 KDa in AWA and both infected and non infectedvisceral hump snail antigens.HIS of B.alexandrina Infected foot detected a common band at 68KDa amongboth infected and non infected foot antigens and AWA. HIS of B. alexandrina non infected visceral hump detecteda common band at 35 KDa among both infected and non infected visceral snail antigens and AWA

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