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Impact of melatonin and cryoprotectants on buck frozen semen during hot and cold months / Elias Michael Gabriel Kodi ; Supervised Gamal Ashour Hassan , Moataz Ahmed Elgayar , Sherif Mohamed Dessouki

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Elias Michael Gabriel Kodi , 2020Description: 89 P. : charts ; 25cmOther title:
  • أثر الميلاتونين والواقيات من البرودة علي السائل المنوى المجمد للتيوس اثناء الشهور الحاره والبارده [Added title page title]
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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Animal Production Summary: This research was designed to assess the improvement of the freezability of buck semen using two different types of cryoprotectants supplemented with melatonin as antioxidant in cold and hot months. Pooled samples from four sexually mature Egyptian Baladi Bucks were used in this experiment. Semen was extended (1:8) with Tris-fructose-citric containing egg yolk using glycerol and dimethyl sulfoxide (DMSO) supplemented with two doses of melatonin (10-6M and10-3M) in addition to control group. Computer assisted semen analysis (CASA) was used to evaluate semen after cryopreservation. While, enzymatic activity was measured using spectrophotometer technique. Real-time PCR was used for expression profile of selected genes.The results revealed that the progressive motility percentage was higher (P<0.05) in samples supplemented with low dose of melatonin (10-6 M) compared to high dose (10-3M) in glycerol (74.4±2.4 vs. 64.4±2.5) and DMSO based extender (35.5 ±2.4 vs. 32.9 ±2.5) in cold months. The same trend was found in samples cryopreserved with glycerol (75.1±2.2 vs. 53.5±2.2) and DMSO (32.1±1.9 vs. 22.0±1.8) in hot months. The results also demonstrated that CASA parameters (VAP and VCL) were significantlyincreased in low compared to high melatonin doses in glycerol based extender during cold and hot months. The activity of total antioxidant capacity (TAC) was significantly higher in samples supplemented with low melatonin dose (0.49 mM/L ±0.09) than high melatonin dose (0.16 mM/L±0.09) in DMSO extender. Transcript abundance of CPT2, ATP5F1A and SOD2 genes was increased significantly in glycerol based extender groups and this was more apparent in low melatonin dose compared with all other glycerol based extender groups in cold monthse post-thaw fertilizing ability of buck semen
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.03.M.Sc.2020.El.I (Browse shelf(Opens below)) Not for loan 01010110081288000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.03.M.Sc.2020.El.I (Browse shelf(Opens below)) 81288.CD Not for loan 01020110081288000

Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Animal Production

This research was designed to assess the improvement of the freezability of buck semen using two different types of cryoprotectants supplemented with melatonin as antioxidant in cold and hot months. Pooled samples from four sexually mature Egyptian Baladi Bucks were used in this experiment. Semen was extended (1:8) with Tris-fructose-citric containing egg yolk using glycerol and dimethyl sulfoxide (DMSO) supplemented with two doses of melatonin (10-6M and10-3M) in addition to control group. Computer assisted semen analysis (CASA) was used to evaluate semen after cryopreservation. While, enzymatic activity was measured using spectrophotometer technique. Real-time PCR was used for expression profile of selected genes.The results revealed that the progressive motility percentage was higher (P<0.05) in samples supplemented with low dose of melatonin (10-6 M) compared to high dose (10-3M) in glycerol (74.4±2.4 vs. 64.4±2.5) and DMSO based extender (35.5 ±2.4 vs. 32.9 ±2.5) in cold months. The same trend was found in samples cryopreserved with glycerol (75.1±2.2 vs. 53.5±2.2) and DMSO (32.1±1.9 vs. 22.0±1.8) in hot months. The results also demonstrated that CASA parameters (VAP and VCL) were significantlyincreased in low compared to high melatonin doses in glycerol based extender during cold and hot months. The activity of total antioxidant capacity (TAC) was significantly higher in samples supplemented with low melatonin dose (0.49 mM/L ±0.09) than high melatonin dose (0.16 mM/L±0.09) in DMSO extender. Transcript abundance of CPT2, ATP5F1A and SOD2 genes was increased significantly in glycerol based extender groups and this was more apparent in low melatonin dose compared with all other glycerol based extender groups in cold monthse post-thaw fertilizing ability of buck semen

Issued also as CD

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