Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

Wastewater and Sludge samples polluted with petroleum oil from Cairo Oil Refining Company (CORC), Mostorod, El-Qalyubiah, Cairo, Egypt were used for isolation of most potent bacterial strains from indigenous bacterial communities. The isolation of bacterial strains followed four steps of adaptation and enrichment technique for selection of the most potent bacterial strains which were capable to degrade the three PAHs Compounds (Naphthalene, Phenenthrene, and Pyrene). Five most potent bacterial isolates (MAM-P9, 14, 22, 25, and 26) were able to grow on Different concentrations of three chosen PAHs and having the abilities to use PAHs as a sole carbon and energy source. The best PAHs (Naphthalene, Phenenthrene, and Pyrene) degraders and Heavy metals uptake bacterial Isolates MAM-14 and MAM-22 were identified by 16S-rRNA and showed a similarity of 99% to Bordetella bronchiseptica strain NBRC 13691 16S ribosomal RNA gene, partial sequence and similarity of 98% to Bordetella avium strain. ATCC 35086 respectively

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