Using isothermal nuleic acid amplification for rapid diagnosis of schistosomaiasis haematobium infection / Angham Mohamed Bayoumi ; Supervised Soad Abdelsalam Alrefaie , Inas Zakria Abdelaziz , Mohamed Sharf Eldin Zaky Badr

Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Parasitology

Urogenital schistosomiasis due to schistosoma haematobium is a serious underestimated public health problem affecting 112 million people particularly in sub saharan Africa. Microscopic examination of urine samples to detect parasite eggs still remains as definitive diagnosis but, this method has low sensitivity, high day to day variability and cannot be carried out in the acute or chronic phase of the disease. Moreover, PCR based technologies are of limited use as it require skilled person and complicated pricy equipment. An interesting approach is the loop-mediated isothermal amplification (LAMP) technique because of its simplicity in operation and potential use in clinical diagnosis and surveillance of infectious diseases. Our study evaluated LAMP technique for S.haematobium DNA detection in 69 urine samples of suspected patients for urogenital schistosomiasis versus ova detection by convential microscopy after urine filtration. The detection limit of our LAMP was 1fg / oL of S. haematobium DNA in urine samples. When testing all patients{u00B4}urine samples included in our study, diagnostic parameters for sensitivity and specificity were calculated for LAMP assay, 100% sensitivity and 63.2% specificity which display that the LAMP technique is a new, simple, sensitive, high through-output diagnostic important tool in clinical diagnosis in poorly equiped facilities as well as in screening for parasitic and infectious diseases

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