The role of microRNA-31 and microRNA-21 as regulator biomarkers in the activation of T-lymphocytes / Eman Eissa Ateya Mohammed ; Supervised Sanaa Osman Abdallah , Faten Sayed Bayoumi , Khalda Said Amr

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry

The present study aimed to evaluate the possibility of using miR-31 and miR- 21 as regulatory biomarkers in the activation of T-lymphocytes of Egyptian lupus patients through estimating the correlation of miR-31 and miR-21 with IL- 2 in SLE patients and investigating the association of miR-31 and miR-21 expressions with the systemic lupus erythematosus disease activity index (SLEDAI) and proteinuria of patients. Quantitative RT-PCR was used to estimate the expression patterns of miR-31 and miR-21, and IL-2 levels were determined using ELISA in plasma of 40 patients with SLE, 20 of their first degree relatives and 20 healthy controls. This study also determined SLEDAI score and proteinuria in SLE patients. The results revealed that miR-31 expression was lower while miR-21 was higher in SLE patients than that of their first degree relatives and normal controls. MiR-31was negatively correlated with SLEDAI and proteinuria in lupus patients while miR-21 showed positively correlation with both of which in those patients. Also the present study found that there is a significant positive correlation between miR-31 and IL-2 in SLE patients while miR-21 was negatively correlated with IL-2 level in those patients. In conclusion, the study disclosed a significant association between miR-31 and miR-21 expressions with IL-2 level in SLE patients. Therefore, both of miR-31 and miR-21 may play an important role as regulatory biomarkers and might have an impact on regulating IL-2 expression and in turn the activation of T-lymphocytes in SLE

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