The immunomodulatory effect of CpG motifs-rich plasmids on immunity developed against Salmonella enterica serovar Enteritidis vaccine in chickens / Mohamed Mohamed Ahmed Soliman Abed ; Supervised Rafik Tawfik Mohamed Soliman , Mahmoud Dardery Mohamed Elhariri

Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology

Bacterial oligodeoxynucleotide containing cytosine guanine motifs (CpG-ODN) has been reported to induce immunostimulatory activity against a variety of bacterial, viral, and protozoan infections in a variety of vertebrate species. The objective of this study was to investigate the dose-dependent immunomodulatory effect of CpG ODN on Salmonella Enteritidis bacterin in broiler chickens. Two hundreds one-day-old broiler chicks, divided into 5 groups, were used in this study. Three groups were immunized with Salmonella Enteritidis bacterin adjuvanted with different doses of CpG ODN (50æg, 100æg and 200æg).The control groups included a group that was immunized with Salmonella Enteritidis bacterin adjuvanted with aluminium hydroxide and a non-immunized group. The intestinal colonization, cellular responses, mucosal and systemic immune responses of immunized chickens were measured at different intervals, until 42 days of age. At two weeks of post-immunization, 20 chicks from each group were orally challenged by Salmonella Enteritidis fresh bacterial culture (1.2x108 CFU/ml).The survival rates and the pathological changes of challenged chickens in the different groups were monitored for extra 10 days. Compared to the aluminium hydroxide adjuvanted bacterin the CpG-ODN adjuvant bacterin induced significant protection and improved survival rate of challenged chickens

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