Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy

Phytochemical study of Phoenix canariensis, includes proximate analysis (to determine percentage of total protein, moisture, total ash, acid insoluble ash and crude fiber). The leaves and pollen grains of the plant were subjected to Preliminary phytochemical screening to test for the presence of different plant constituents. Study of lipoidal matter of the leaves and pollen grains by GLC analysis of unsaponifiable matters as well as the fatty acid methyl esters (FAME) The study revealed that the phytosterols percent is much higher in the pollen grains than in the leaves and the identified compounds of the fatty acids in leaves and pollen grains was 17 and 19, respectively. Investigation of the ethyl acetate fractions of the leaves and pollen grains that afforded two compounds was isolated. Investigation of the butanol fractions of the leaves and pollen grains that afforded five compounds was isolated. The HPLC analysis of the leaves and pollen grains, separately, revealed the presence of 18 phenolic compounds and 10 flavonoids. Spectrophotometric estimation of the major classes (steroids, polyphenolics, tannins and flavonoids) of constituents of the leaves and pollen grains. Estimation of saponin content was done by blood agar diffusion method. Spathe volatiles analysis using solid phase microextraction coupled with GC/MS that revealed the presence of 52 compounds Nutritional evaluation of the pollen grains that including the investigation of carbohydrates, proteins and amino acids and HPLC analysis of vitamins content (vitamin A, E and C). The pollen grains contain 16 amino acids. Lysine was the highest percent of amino acids in pollen grains. Biological study of Phoenix canariensis, that including determination of median lethal dose (LD ), anti-hyperglycemic activity, acute anti-inflammatory activity, antioxidant activity, 50 hepatoprotective activity, cytotoxic activity, histopathological study of pollen grains on benign prostatic hyperplasia and on stressed testicular tissue and antimicrobial activity

Issued also as CD