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Physiological and molecular studies associated with engineering dehydration- stress tolerance in sesame / Amal Farouk Abdelhamied Alshafeay ; Supervised Mohamed Ramadan Aboulella Nesiem , Ahmed Shawky Ibrahim , Mohamed Saleh Tawfik

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Amal Farouk Abdelhamied Alshafeay , 2018Description: 120 P. : photographs ; 25cmOther title:
  • دراسات فسيولوجيه و جزيئيه على نباتات السمسم المهندسه وراثيا لمقاومه الإجهاد بالجفاف [Added title page title]
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  • Issued also as CD
Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Botany Summary: Drought is a major stress that globally restricting the agricultural expansion and crops productivity. Sesame (Sesamum indicum L.) considered as a recalcitrant plant to be regenerated. In this study for improving the dehydration stress tolerance of sesame plant, a LOS5 of Arabidopsis thaliana was introduced to de-embryonated cotyledons of sesame cv. Sohag1 via Agrobacterium tumefaciens strain LBA4404 which harboring the LOS5 gene encode a molybdenum cofactor sulfurase (MCSU), which catalyzes the generation of the sulfurylated form of MoCo, a cofactor required by aldehyde oxidase to functions in the last step of ABA biosynthesis, leading to ABA accumulation in tissues followed by enhancing the drought stress tolerance. The insertion of AtLOS5 was confirmed by PCR and RT-PCR analysis
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.05.Ph.D.2018.Am.P (Browse shelf(Opens below)) Not for loan 01010110078630000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.07.05.Ph.D.2018.Am.P (Browse shelf(Opens below)) 78630.CD Not for loan 01020110078630000

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Botany

Drought is a major stress that globally restricting the agricultural expansion and crops productivity. Sesame (Sesamum indicum L.) considered as a recalcitrant plant to be regenerated. In this study for improving the dehydration stress tolerance of sesame plant, a LOS5 of Arabidopsis thaliana was introduced to de-embryonated cotyledons of sesame cv. Sohag1 via Agrobacterium tumefaciens strain LBA4404 which harboring the LOS5 gene encode a molybdenum cofactor sulfurase (MCSU), which catalyzes the generation of the sulfurylated form of MoCo, a cofactor required by aldehyde oxidase to functions in the last step of ABA biosynthesis, leading to ABA accumulation in tissues followed by enhancing the drought stress tolerance. The insertion of AtLOS5 was confirmed by PCR and RT-PCR analysis

Issued also as CD

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