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Multiplex PCR for detection of five selected carbapenemase genes in comparative study with modified-hodge test in gram-negative bacilli isolated from healthcare-associated infections among critically Ill patients / Ahmed Salah Emira Aly ; Supervised Abdelfattah Mohamed Attia , Reham Ali Dwedar , Nazmy Edward Seif

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Cairo : Ahmed Salah Emira Aly , 2018Description: 118 P. : charts ; 25cmOther title:
  • تفاعل البلمرة المتسلسل المتعدد للكشف عن خمسة جينات للكاربابينيماز في دراسة مقارنة مع اختبار هودج المعدّل في البكتيريا سالبة الجرام المعزولة من حالات العدوى المرتبطة بالرعاية الصحية بين مرضى الحالات الحرجة [Added title page title]
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Dissertation note: Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology Summary: Background:Carbapenemase producing Gram-negative bacilli have been reported to be an important cause of hospital associated infections (HAIs), especially in critically ill patients with device associated infections (DAIs). Aim of the work: To estimate the extent of carbapenemase producing Gram-negative bacteria in critically ill patients from Kasr El-Aini, Cairo University hospitals and to compare the Modified-Hodge test (MHT) as a phenotypic confirmatory test with the PCR as a gold standard genotypic method for their diagnosis. Patients and methods:95 bacterial isolates from 75 critically ill patients were collected over a period of six months from November 2016 to April 2017 from Cairo university hospitals. These isolates were subjected to susceptibility testing for carbapenems as per CLSI guidelines and were further phenotypically screened for the production of carbapenemase by MHT, then were subjected to genotypic analysis by multiplex PCR
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Thesis Thesis قاعة الرسائل الجامعية - الدور الاول المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.Ph.D.2018.Ah.M (Browse shelf(Opens below)) Not for loan 01010110078603000
CD - Rom CD - Rom مخـــزن الرســائل الجـــامعية - البدروم المكتبة المركزبة الجديدة - جامعة القاهرة Cai01.11.19.Ph.D.2018.Ah.M (Browse shelf(Opens below)) 78603.CD Not for loan 01020110078603000

Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology

Background:Carbapenemase producing Gram-negative bacilli have been reported to be an important cause of hospital associated infections (HAIs), especially in critically ill patients with device associated infections (DAIs). Aim of the work: To estimate the extent of carbapenemase producing Gram-negative bacteria in critically ill patients from Kasr El-Aini, Cairo University hospitals and to compare the Modified-Hodge test (MHT) as a phenotypic confirmatory test with the PCR as a gold standard genotypic method for their diagnosis. Patients and methods:95 bacterial isolates from 75 critically ill patients were collected over a period of six months from November 2016 to April 2017 from Cairo university hospitals. These isolates were subjected to susceptibility testing for carbapenems as per CLSI guidelines and were further phenotypically screened for the production of carbapenemase by MHT, then were subjected to genotypic analysis by multiplex PCR

Issued also as CD

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