Studies on methods used for virus detection from aquatic environment by cell culture and polymerase chain reaction / Ashraf Roshdy Saleh Mahmoud Shoeib ; supervised Mohamed Ibrahim Ali , Shawki El - Said El - Hawaary ,

Thesis (PH.D.) - Cairo University - Faculty Of Science - Department Of Botany (Microbiology)

Waterborne viral infections are increasingly recognized as causes of illness in humansThis increase is partly explained by changes in water quality and consumption patterns that lead to the worldwide availability of high - riskIn our work we studied the using of two methods to achieve our aimsThe first method was cell culture method representing in plaque infectivity assay technique for counting viruses to achieve our goal for testing of terrestrial and marine inexpensive wastes and their derivatives for evaluation of these materials and their derivatives for virus removal from waterThe second method was polymerase chain reaction methods for study molecular changes and characterization of highly passaged cell culture adapted hepatitis A virus

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