000 034290000a22003490004500
003 EG-GICUC
005 20250223025344.0
008 061104s2005 ua a f m 000 0 eng d
040 _aEG-GICUC
_beng
_cEG-GICUC
041 0 _aEng
049 _aDeposite
097 _aPh.D
099 _aCai01.07.16.Ph.D.2005.Ma.S.
100 0 _aManal Aly ElShazly Aly
245 1 0 _aStudies on a virus infecting sugar beet plants in Egypt /
_cManal Aly ElShazly Aly ; supervised Aly Mohamed Mamoun Abd El Salam , Said Mohamed Aboul Ela , Moustafa Ahmed ElKady
246 1 5 _aدراسات على احد الفيروسات التى تصيب نباتات بنجر السكر فى مصر
260 _aCairo :
_bManal Aly ElShazly Aly ,
_c2005
300 _a200P :
_bill ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty Of Agriculture - Department Of Plant Pathology and Physiology
520 _aA new Ilravirus infecting sugar beet plants was isolated for the first time in EgyptThe virus was given the provisional name beet necrotic ringspot virus BNRSV - EGBNRSV - EG caused ring spot , line pattern and calico mosaic on sugar beet in the fieldIt infected several hosts belonging to seven familiesBNRSV - EG was transmitted mechanically and through Thrips tabaci insects , but not through Myzus persicae aphids or through the tested seeds of some sugar beet cultivarsNine commercial cvs were found to be susceptible to BNRSV - EGccording to indirect ELISA testVirus infection increased the ? - amino nitrogen , moisture , sodium , potassium content and sugar loss in molasses in all tested sugar beet roots infected with BNRSV - EG compared with healthy root plantsOn the other hand , virus infection decreased the sugar purity , polarization , sugar extractability , sugar extract and protein content of infected sugar beet roots compared with healthy rootsThe incidence of sugar beet infection with BNRSV - EG in this study rated 4125and 34 in the tested field in Kafr El - Sheikh and El - Fayoum , respectivelyPurified BNRSV - EG migrated in sucrose gradient forming three components with average A260 / 280 of 15 and 163 for un - fractionated and fractionated components , respectivelyCoat protein subunits of BNRSV - EG had a value of 29 KDaGenomic RNAs were separated into four species of RNAs in agarose gel electrophoresisSeveral serologic tests indicated its close ties with the Ilarvirus , Prunus necrotic ringspot virus , PNRSVMolecular studies showed the ability of the universal Jc 10 / Jc 12 primer pairs for PNRSV to amplify 641 bp of the coat protein gene of both PNRSV and BNRSV - EG further , BNRSV - EG coat protein gene had a Mse 1 restriction site similar to the sever isolates of PNRSVNucleotide sequence analysis of the 641pb amplified - coat protein gene of BNRSV - EG indicated 6580 similarities with the published sequences of IlarvirusesSequence relationship between BNRSV - EG isolate and PNRSV CH3 , CH19 , ring 21 and ring 28 isolates was 9315 , 9103 , 9047 and 8914
530 _aIssued also as CD
653 4 _aBeet plants
653 4 _aEgypt
653 4 _aVirus infecting sugar
700 0 _aAly Mohamed Mamoun Abd El Salam ,
_esupervisor
700 0 _aMoustafa Ahmed ElKady ,
_esupervisor
700 0 _aSaid Mohamed Aboul Ela ,
_esupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aEnas
_eCataloger
905 _aMustafa
_eRevisor
942 _2ddc
_cTH
999 _c11171
_d11171