000			07007namaa22004091i 4500
003			OSt
005			20240211192451.0
008			231119s2023 ua a|||frm||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a591.35 _221
092			_a591.35 _221
097			_aM.Sc
099			_aCai01.12.21.M.Sc.2023.Re.E
100	0		_aReem Maged Ahmed Sheta, _epreparation.
245	1	0	_aEstimation of the safety and antitumor activity of silver doped hydroxyapatite nanoparticles in mice / _cby Reem Maged Ahmed Sheta ; supervised by Ass. Prof. Hanan Ramadan Hamad, Prof. Gehan Safwat ElHusseiny.
246	1	5	_aﺗﻘدﯾر اﻟﺳﻼﻣﺔ واﻟﻧﺷﺎط اﻟﻣﺿﺎد ﻟﻠورم ﻣن ﺟزﯾﺋﺎت ھﯾدروﻛﺳﯾﺑﺎﺗﯾت اﻟﻧﺎﻧوﯾﺔ اﻟﻣطﻌﻣﺔ ﺑﺎﻟﻔﺿﺔ ﻓﻲ اﻟﻔﺋران
264		0	_c2023.
300			_a135 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rdacontent
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (M.Sc.)-Cairo University, 2023.
504			_aBibliography: pages 119-131.
520			_aCurrently, the main therapies for treating cancer are associated with a variety of side effects. Accordingly, researchers are focusing on the use of nanotherapy to develop a highly novel, less toxic and extremely efficient therapeutic agents for cancer treatment. Silver doped Hydroxyapatite nanoparticles (Ag:HAp-NPs) have shown effective at treating both infections and cancerous tumors while being non- cytotoxic to normal healthy cells in vitro. Therefore, this study was undertaken to estimate the safety and anti-tumor activity of silver doped hydroxyapatite nanoparticles in mice in vivo. Regarding the antitumor activity, concurrent administration of Ag:HAp-NPs caused a significant decrease in tumor volume and weight. Additionally, it showed a high elevation of intracellular ROS generation as well as MDA levels within tumor tissues and lead to the induction of DNA breaks. The high production of intracellular ROS generation, and the oxidative stress induction by Ag:HAp-NPs within tumor tissues confirmed a decrease in GSH and GPx levels. Significant elevation of P53 and HO-1 gene and decrease in Hsp70 and Kras was observed as well. Additionally, the percentage of necrotic tissue in tumor- induced groups and % of degenerated tumor cells after treatment with Ag:HAp-NPs were significantly elevated. Regarding the safety of Ag:HAp- NPs, it was shown to be safe and non-genotoxic. This was confirmed by the non-significant changes in TL and TM in the bone marrow and liver tissues. It was further confirmed by the observed non- significant changes in the MDA and TAC levels as well as the SOD activity in the liver among the safety groups, except for high dosage level. Moreover, histological examinations demonstrated that Silver doped Hydroxyapatite nanoparticles have mild toxicity on kidney and liver tissues with minimal inflammatory reactions associated with the presence of Ag:HAp-NPs in contrast with Cisplatin. In conclusion, silver doped hydroxyapatite nanoparticles have a potential anticancer activity against induced solid tumors in mice and demonstrated a higher level of safety for the liver, kidney and bone marrow tissues, making Ag:HAp-NPs a promising anti-tumor agent.
520			_aﺣﺎﻟًﯿﺎ،ﺗﺮﺗﺒﻂ اﻟﻌﻼﺟﺎت اﻟﺮﺋﯿﺴﯿﺔ ﻟﻌﻼج اﻟﺴﺮطﺎن ﺑﻤﺠﻤﻮﻋﺔ ﻣﺘﻨﻮﻋﺔﻣﻦاﻵﺛﺎر اﻟﺠﺎﻧﺒﯿﺔ.وﻓًﻘﺎ ﻟﺬﻟﻚ،ﯾﺮﻛﺰا ﻟﺒﺎﺣﺜﻮن ﻋﻠﻰ اﺳﺘﺨﺪام اﻟﻌﻼج ﺑﺎﻟﻨﺎﻧﻮ ﻟﺘﻄﻮﯾﺮ ﻋﻮاﻣﻞ ﻋﻼﺟﯿﺔ ﺟﺪﯾﺪة ﻟﻠﻐﺎﯾﺔ ؤاﻗﻞ ﺳﻤﯿﺔ وﻓﻌﺎﻟﺔ ﻟﻠﻐﺎﯾﺔ ﻟﻌﻼج اﻟﺴﺮطﺎن.ٔاظﮭﺮت ﺟﺰﯾﺌﺎت ھﯿﺪروﻛﺴﯿﺒﺎﺗﯿﺖ اﻟﻨﺎﻧﻮﯾﺔ اﻟﻤﻄﻌﻤﺔ ﺑﺎﻟﻔﻀﺔ)Ag: HAp-NPs( ﻓﻌﺎﻟﯿﺘﮭﺎ ﻓﻲ ﻋﻼج ﻛﻞ ﻣﻦ اﻻﻟﺘﮭﺎﺑﺎت واﻷورام اﻟﺴﺮطﺎﻧﯿﺔ ﺑﯿﻨﻤﺎ ﺗﻜﻮن ﻏﯿﺮﺳﺎﻣﺔ ﻟﻠﺨﻼﯾﺎ اﻟﺴﻠﯿﻤﺔ ﻣﻌﻤﻠﯿﺎ in vitro .ﻟﺬﻟﻚ،ٔ اﺟﺮﯾﺖ ھﺬه اﻟﺪراﺳﺔ ﻟﺘﻘﺪﯾﺮ اﻟﺴﻼﻣﺔ واﻟﻨﺸﺎط اﻟﻤﻀﺎد ﻟﻠﻮرم ﻣﻦ ﻟﺠﺴﯿﻤﺎت ھﯿﺪروﻛﺴﯿﺒﺎﺗﯿﺖ اﻟﻨﺎﻧﻮﯾﺔ اﻟﻤﻄﻌﻤﺔ ﺑﺎﻟﻔﻀﺔ ﻓﻲ اﻟﻔﺌﺮان ﻓﻲ اﻟﺠﺴﻢ اﻟﺤﻲ in vivo .ﻓﯿﻤﺎ ﯾﺘﻌﻠﻖ ﺑﺎﻟﻨﺸﺎط اﻟﻤﻀﺎد ﻟﻠﻮرم، ﺗﺴﺒﺐ اﻹ ﻋﻄﺎء اﻟﻤﺘﺰاﻣﻦ ﻟـ Ag: HAp-NPs ﻓﻲ اﻧﺨﻔﺎض ﻛﺒﯿﺮ ﻓﻲ ﺣﺠﻢ ووزن اﻟﻮرم. ﺑﺎﻹﺿﺎﻓﺔٕ اﻟﻰ ذﻟﻚ اظﮭﺮ ارﺗﻔﺎﻋﺎ ﻛﺒیرا ﻓﻲﺗﻮﻟﯿﺪ ROSداﺧﻞ اﻟﺨﻼﯾﺎ وﻛﺬﻟﻚ ﻣﺴﺘﻮﯾﺎتMDA داﺧل أﻧﺴﺠﺔ اﻟﻮرم وﯾٔﻮديٕ اﻟﻰ ﺗﺤﺮﯾﺾ ﻓﻮاﺻﻞ اﻟﺤﻤﺾاﻟﻨﻮوي.ٔ اﻛﺪ اﻹﻧﺘﺎج اﻟﻌﺎﻟﻲﻟﺘﻮﻟﯿﺪ ROS داﺧﻞ اﻟﺨﻼﯾﺎ، وﺗﺤﺮﯾﺾ اﻹﺟﮭﺎد اﻟﺘﺄﻛﺴﺪي ﺑﻮاﺳﻄﺔ Ag: HAp-NPs داﺧﻞٔ اﻧﺴﺠﺔاﻟﻮرم اﻧﺨﻔﺎﺿا ﻓﻲ ﻣﺴﺘﻮﯾﺎت GSHو.GPxﻛﻤﺎ ﻟﻮﺣﻆ ارﺗﻔﺎع ﻛﺒﯿﺮ ﻓﻲ ﺟﯿﻦP53وHO-1واﻧﺨﻔﺎضﻓﻲ Hsp70وKras.ﺑﺎﻹﺿﺎﻓﺔٕ اﻟﻰذﻟﻚ،ﺗﻢ رﻓﻊ ﻧﺴﺒﺔ اﻷﻧﺴﺠﺔ اﻟﻤﯿﺘﺔ ﻓﻲ اﻟﻤﺠﻤﻮﻋﺎت اﻟﺘﻲ ﯾﺴﺒﺒﮭﺎ اﻟﻮرم وﻧﺴﺒﺔ اﻟﺨﻼﯾﺎ اﻟﺴﺮطﺎﻧﯿﺔاﻟﻤﺘﺪھﻮرةﺑﻌﺪاﻟﻌﻼج Ag: HAp-NPsﺑﺸﻜﻞﻣﻠﺤﻮظ.ﻓﯿﻤﺎﯾﺘﻌﻠﻖﺑﺴﻼﻣﺔ :Ag HAp-NPs،ﻓﻘﺪﺛﺒﺖٔ اﻧﮭﺎ ٓاﻣﻨﺔوﻏﯿﺮﺳﺎﻣﺔﻟﻠﺠﯿﻨﺎت.ﺗﻢﺗﺄﻛﯿﺪذﻟﻚﻣﻦﺧﻼل اﻟﺘﻐﯿﯿﺮات ﻏﯿﺮاﻟﻤﮭﻤﺔﻓﻲTLوTMﻓﻲﻧﺨﺎع اﻟﻌﻈﺎم ؤاﻧﺴﺠﺔاﻟﻜﺒﺪ.ﺗﻢ ﺗﺄﻛﯿﺪذﻟﻚٔای ًﺿﺎﻣﻦ ﺧﻼل اﻟﺘﻐﯿﯿﺮات ﻏﯿﺮاﻟﻤﻠﺤﻮظﺔ ﻓﻲﻣﺴﺘﻮﯾﺎت MDAوTACﺑﺎﻹﺿﺎﻓﺔٕ اﻟﻰﻧﺸﺎطSODﻓﻲاﻟﻜﺒﺪ
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aGenetics
653		0	_aSilver doped hydroxyapatite _acancer _amice _aantitumor _aoxidative stress and safety
700	0		_aHanan Ramadan Hamad, _ethesis advisor.
700	0		_aGehan Safwat El Husseiny, _ethesis advisor.
900			_b28-09-2023 _cHanan Ramadan Hamad _cGehan Safwat El Husseiny _dAhmed Emam Dakroury _UCairo University _FFaculty of Science _DDepartment of Zoology
905			_aHanan
942			_2ddc _cTH _e21 _n0
999			_c165719 _d165719