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008 | 241120s2023 |||a|||fr|m|| 000 0 eng d | ||
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_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda |
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041 | 0 |
_aeng _beng _bara |
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049 | _aDeposit | ||
082 | 0 | 4 | _a580 |
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097 | _aM.Sc | ||
099 | _aCai01.12.05.M.Sc.2023.Qi.P | ||
100 | 0 |
_aGihad Ahmed Sayed Mohammed, _epreparation. |
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245 | 1 | 0 |
_aPurification, characterization, and molecular studies on S- adenosyl methionine synthase in dimorphic fungi / _cby Gihad Ahmed Sayed Mohammed ; supervisors: by Prof. Dr. Mohsen A. Sayed, Dr. Eman Abdullah M. Ali |
246 | 1 | 5 | _aتنقيه, توصيف, و دراسات جزيئيه للإنزيم المصنع للادينوسايل ميثيونين داخل الفطريات ثنائية الشكل / |
264 | 0 | _c2023. | |
300 |
_a112 pages : _billustrations ; _c25 cm. + _eCD. |
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336 |
_atext _2rda content |
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337 |
_aUnmediated _2rdamedia |
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338 |
_avolume _2rdacarrier |
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502 | _aThesis (M.Sc.)-Cairo University, 2023. | ||
504 | _aBibliography: pages 77-112. | ||
520 | _aCandida spp. was kindly supplied by the micro-analytical center Cairo University. Identification took place biochemically and molecularly. biochemically using the Vitek2 technique, and CHROM-agar media, and molecularly through its DNA extraction, and sequencing. C. albicans was sub-cultured and purified on saboraud–dextrose agar (SDA), yeast agar (YA), and Potatoe dextrose agar (PDA) , while dimorphism phenomenon was enhanced through sub-culturing on RPMI1640 growing media, and then dimorphism was monitored using light microscope, with the estimation of the amount of SAM produced in the yeast cell using HPLC to reflect elevation or depression in the S-adenosyl methionine synthase enzyme. Adomet synthase, extracted and purified from C. albicans, and then activity assay was performed to get well known to the optimal and minimal conditions of the enzyme activity, and the factors that affect its activity. Identifying, and DNA sequencing for the gene that responsible for the enzyme production from C. albicans strain. | ||
530 | _aIssued also as CD | ||
546 | _aText in English and abstract in Arabic & English. | ||
650 | 7 |
_aBotany and Microbiology _2qrmak |
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653 | 0 |
_aCandida albicans _aSAM _aS-adenosyl methionine synthase _aAdomet |
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700 | 0 |
_aMohsen A. Sayed _ethesis advisor. |
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700 | 0 |
_aEman Abdullah M. Ali _ethesis advisor. |
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_b01-01-2023 _cMohsen A. Sayed _cEman Abdullah M. Ali _UCairo University _FFaculty of Science _DDepartment of Botany and Microbiology |
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_aEman _eHuda |
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_2ddc _cTH _e21 _n0 |
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999 | _c168964 |