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003			OSt
005			20250223033328.0
008			241120s2023 |||a|||fr|m|| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a580
092			_a580 _221
097			_aM.Sc
099			_aCai01.12.05.M.Sc.2023.Qi.P
100	0		_aGihad Ahmed Sayed Mohammed, _epreparation.
245	1	0	_aPurification, characterization, and molecular studies on S- adenosyl methionine synthase in dimorphic fungi / _cby Gihad Ahmed Sayed Mohammed ; supervisors: by Prof. Dr. Mohsen A. Sayed, Dr. Eman Abdullah M. Ali
246	1	5	_aتنقيه, توصيف, و دراسات جزيئيه للإنزيم المصنع للادينوسايل ميثيونين داخل الفطريات ثنائية الشكل /
264		0	_c2023.
300			_a112 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (M.Sc.)-Cairo University, 2023.
504			_aBibliography: pages 77-112.
520			_aCandida spp. was kindly supplied by the micro-analytical center Cairo University. Identification took place biochemically and molecularly. biochemically using the Vitek2 technique, and CHROM-agar media, and molecularly through its DNA extraction, and sequencing. C. albicans was sub-cultured and purified on saboraud–dextrose agar (SDA), yeast agar (YA), and Potatoe dextrose agar (PDA) , while dimorphism phenomenon was enhanced through sub-culturing on RPMI1640 growing media, and then dimorphism was monitored using light microscope, with the estimation of the amount of SAM produced in the yeast cell using HPLC to reflect elevation or depression in the S-adenosyl methionine synthase enzyme. Adomet synthase, extracted and purified from C. albicans, and then activity assay was performed to get well known to the optimal and minimal conditions of the enzyme activity, and the factors that affect its activity. Identifying, and DNA sequencing for the gene that responsible for the enzyme production from C. albicans strain.
530			_aIssued also as CD
546			_aText in English and abstract in Arabic & English.
650		7	_aBotany and Microbiology _2qrmak
653		0	_aCandida albicans _aSAM _aS-adenosyl methionine synthase _aAdomet
700	0		_aMohsen A. Sayed _ethesis advisor.
700	0		_aEman Abdullah M. Ali _ethesis advisor.
900			_b01-01-2023 _cMohsen A. Sayed _cEman Abdullah M. Ali _UCairo University _FFaculty of Science _DDepartment of Botany and Microbiology
905			_aEman _eHuda
942			_2ddc _cTH _e21 _n0
999			_c168964