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040 _aEG-GICUC
_beng
_cEG-GICUC
_dEG-GICUC
_erda
041 0 _aeng
_beng
_bara
049 _aDeposit
082 0 4 _a580
092 _a580
_221
097 _aM.Sc
099 _aCai01.12.05.M.Sc.2023.Qi.P
100 0 _aGihad Ahmed Sayed Mohammed,
_epreparation.
245 1 0 _aPurification, characterization, and molecular studies on S- adenosyl methionine synthase in dimorphic fungi /
_cby Gihad Ahmed Sayed Mohammed ; supervisors: by Prof. Dr. Mohsen A. Sayed, Dr. Eman Abdullah M. Ali
246 1 5 _aتنقيه, توصيف, و دراسات جزيئيه للإنزيم المصنع للادينوسايل ميثيونين داخل الفطريات ثنائية الشكل /
264 0 _c2023.
300 _a112 pages :
_billustrations ;
_c25 cm. +
_eCD.
336 _atext
_2rda content
337 _aUnmediated
_2rdamedia
338 _avolume
_2rdacarrier
502 _aThesis (M.Sc.)-Cairo University, 2023.
504 _aBibliography: pages 77-112.
520 _aCandida spp. was kindly supplied by the micro-analytical center Cairo University. Identification took place biochemically and molecularly. biochemically using the Vitek2 technique, and CHROM-agar media, and molecularly through its DNA extraction, and sequencing. C. albicans was sub-cultured and purified on saboraud–dextrose agar (SDA), yeast agar (YA), and Potatoe dextrose agar (PDA) , while dimorphism phenomenon was enhanced through sub-culturing on RPMI1640 growing media, and then dimorphism was monitored using light microscope, with the estimation of the amount of SAM produced in the yeast cell using HPLC to reflect elevation or depression in the S-adenosyl methionine synthase enzyme. Adomet synthase, extracted and purified from C. albicans, and then activity assay was performed to get well known to the optimal and minimal conditions of the enzyme activity, and the factors that affect its activity. Identifying, and DNA sequencing for the gene that responsible for the enzyme production from C. albicans strain.
530 _aIssued also as CD
546 _aText in English and abstract in Arabic & English.
650 7 _aBotany and Microbiology
_2qrmak
653 0 _aCandida albicans
_aSAM
_aS-adenosyl methionine synthase
_aAdomet
700 0 _aMohsen A. Sayed
_ethesis advisor.
700 0 _aEman Abdullah M. Ali
_ethesis advisor.
900 _b01-01-2023
_cMohsen A. Sayed
_cEman Abdullah M. Ali
_UCairo University
_FFaculty of Science
_DDepartment of Botany and Microbiology
905 _aEman
_eHuda
942 _2ddc
_cTH
_e21
_n0
999 _c168964