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008			241223s2023 |||a|||f |m|| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a616.07
092			_a616.07 _221
097			_aM.Sc
099			_aCai01.11.07.M.Sc.2023.Om.S.
100	0		_aOmnia Attia Saad, _epreparation.
245	1	0	_aStudy of the association between SIRT1 single nucleotide gene variant and corticosteroid response in Egyptian pediatric patients with primary immune thrombocytopenia / _cBy Omnia Attia Saad; Supervised By Prof. Dr. Nevine Mohamed Bahaa Eldin Fouad, Asst. Prof. Marwa Abd Elhady Abd Elsamad, Dr. Doaa Salah Mahmoud
246	1	5	_aفى الاطفال SIRT 1 دراسة ارتباط تعدد الجينى لجين المصرين المصابين بمرض نقص الصفائح الدموية المناعى ومدي الاستجابه للعلاج بالكورتيزون /
264		0	_c2023.
300			_a93 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (M.Sc.) -Cairo University, 2023.
504			_aBibliography: pages 76-90.
520			_aBackground: Primary immune thrombocytopenia (ITP) is an autoimmune disorder characterized by decreased platelet count. While corticosteroids are a useful first- line therapy for ITP patients, their long-term effectiveness is limited, and the determinants of corticosteroid sensitivity in ITP patients remain largely unknown. Silent information regulator 1 (SIRT1) is a histone deacetylase of nicotinamide adenine dinucleotide (NAD+), which mainly exists in the nucleus, and is a member of a family of well-studied mammalian sirtuins. SIRT1 interacts with protein substrates in a variety of signaling pathways (such as Wnt and Notch), participates in the regulation of most of the body’s physiological functions, and plays a central regulatory role in cell proliferation, differentiation, senescence, apoptosis, and metabolism.it is also related to the anti-inflammatory effect of corticosteroids. Aim of the Work: The present work was done to study the prevalence and role of SIRT1 SNP rs12778366 in ITP susceptibility and corticosteroids sensitivity. Participants and Methods: Sixty patients with ITP as well as 60 age and sex- matched normal healthy controls were included. SIRT1 rs12778366 SNP was determined by allelic discrimination Taqman real-time PCR. Results: Our study revealed that SIRT1 rs12778366 variants among ITP patients were found to be the wild type T/T (81.7%), the heterozygous T/C (16.7%) and the homozygous C/C genotype (1.7%), while among the control group were the wild type T/T (90%), the heterozygous T/C (10%) and non were in the homozygous C/C with no statistically significant difference between the 2 groups (p ˃0.05) and that SIRT1 rs12778366 variants had no role as a risk factor of ITP susceptibility. Regarding sensitivity to corticosteroids, patients who were sensitive to steroids were found to be the wild type T/T (90%), the heterozygous T/C (10%) and non were in the homozygous C/C, while ITP patients who were resistant to corticosteroids, the wild type T/T (73.3%), the heterozygous T/C (23.3%) and the homozygous C/C (3.3%) with no significant difference between the 2 groups (p ˃0.05) and that SIRT1 rs12778366 variants had no impact on prognosis or treatment outcome. Conclusion: SIRT1 rs12778366 had no role in ITP susceptibility, disease severity or corticosteroid sensitivity.
530			_aIssued also as CD
546			_aText in English and abstract in Arabic & English.
650		7	_aPathology _2qrmak
653		0	_aImmune thrombocytopenic purpura _aSIRT1 rs12778366 _agene variants _aTaqman RT-PCR
700	0		_aNevine Mohamed Bahaa Eldin Fouad _ethesis advisor.
700	0		_aMarwa Abd Elhady Abd Elsamad _ethesis advisor.
700	0		_aDoaa Salah Mahmoud _ethesis advisor.
900			_b01-01-2023 _cNevine Mohamed Bahaa Eldin Fouad _cMarwa Abd Elhady Abd Elsamad _cDoaa Salah Mahmoud _UCairo University _FFaculty of Medicine _DDepartment of Clinical and Chemical Pathology
905			_aNourhan _eHuda
942			_2ddc _cTH _e21 _n0
999			_c169673