000			03022cam a2200325 a 4500
003			EG-GiCUC
008			141118s2014 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.13.Ph.D.2014.Ha.M
100	0		_aHanaa Mohamed Mohamed Elzahed
245	1	0	_aMolecular identification of blood stains on different surfaces and fabrics using specific multiplex mRNA / _cHanaa Mohamed Mohamed Elzahed ; Supervised Osama Mohamed Ebrahim Albarany , Amany Fahmy Hanon , Dina Sabry Abdelfatah
246	1	5	_aالاستعراف الجزيئي لبقع الدم علي الأسطح و الأنسجة المختلفة باستخدام التفاعل المتعدد المحدد للحمض النووي الريبوزى الراسل
260			_aCairo : _bHanaa Mohamed Mohamed Elzahed , _c2014
300			_a123 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine and Clinical Toxicology
520			_a In current forensic practice, blood stain identification is determined using enzymatic, immunologic and chemical testing. Recently, mRNA-profiling has emerged as an alternative strategy to identify the blood stains. mRNA profile clearly reveals tissue-specific gene expression patterns. HBB and HBA (the alpha and beta subunits of hemoglobin A) are specific mRNA blood biomarkers. This study aimed at identification of blood stains on different tested surfaces and fabrics for forensic application by molecular assessment of specific mRNA blood biomarkers. For this study, 100 blood samples were collected from healthy volunteers and they were classified into: (1) 9 groups, each group included 10 samples that were applied on 9 different fabrics and surfaces; cotton, wool, silk, synthetic nylon, wood, glass, ceramic, iron as a metal and leather. (2) One blood group included 10 samples that were applied on glass and exposed to humidity and high temperature to test their effect on mRNA. Ten saliva samples were collected from healthy volunteers and were applied on glass as a negative control body fluid. All samples were left to dry for 1 day. The next steps of the study were RNA extraction from blood and saliva dried stains, reverse transcription, performing gene expression analysis of the blood specific markers using RT-PCR then detection of the amplified gene using agarose gel electrophoresis. The results demonstrated that HBB and HBA can be recovered from blood stains in sufficient quantity and quality for mRNA analysis
530			_aIssued also as CD
653		4	_aBlood stains
653		4	_aBody fluid identification
653		4	_aglobin genes
700	0		_aAmany Fahmy Hanon , _eSupervisor
700	0		_aDina SabryAbdelfatah , _eSupervisor
700	0		_aOsama Mohamed Ebrahim Albarany , _eSupervisor
905			_aAml _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c48274 _d48274