000			02405cam a2200337 a 4500
003			EG-GiCUC
005			20250223031135.0
008			150120s2014 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.05.M.Sc.2014.Ma.P
100	0		_aMai Anwar Zaki Mohamed
245	1	0	_aProduction of immobilized cellulase enzyme by some microorganisms from the rice straw agro-waste using Þ-irradiation / _cMai Anwar Zaki Mohamed ; Supervised Zeinat Kamel Mohamed , Abeer Abdelhady Ahmed
246	1	5	_aإنتاج إنزبم السيلوليز المقيد بواسطة بعض الميكروبات من المخلف الزراعى قش الأرز بإستخدام الأشعاع الجامي
260			_aCairo : _bMai Anwar Zaki Mohamed , _c2014
300			_a158 P. : _bchaers,facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science- Department of Botany Microbiology
520			_aStudies were carried out using 14 fungal cultures screened for their ability to produce cellulase enzymes. A .hortai was selected for the present research as a potent cellulase producer. Cultural and nutritional factors affecting cellulase production were also investigated in order to optimize the fermentation conditions for the maximization of production. The obtained results revealed that, the maximum cellulase production (0.23 U/ml) was achieved after 96 h in a liquid medium (PH 7.0) inoculated with 10% v/v inoculums size, at temperature 37 {u00BA}C, containing (gL⁻¹) CMC, 5.0; yeast extract, 0.1; (NH₄)₂SO₄, 0.5; KH₂PO₄, 10.0; MgSO₄.7H₂O, 0.1 and NaCl, 0.2. The activity remained almost stable between pH 6.0 and 7.0. The highest cellulase activity (1.18 U/ml) was obtained at a lactose concentration of (5.0 g L⁻¹). Partial purification of the crude cellulase by ammonium sulphate 70% saturation showed the highest specific enzyme activity and purification fold (2.3 U/mg protein and 2.12 fold, respectively)
530			_aIssued also as CD
653		4	_aCellulase
653		4	_aImmobilization
653		4	_aRice straw
700	0		_aAbeer Abdelhady Ahmed , _eSupervisor
700	0		_aZeinat Kamel Mohamed , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAml _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c49209 _d49209