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008 150312s2014 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.09.02.Ph.D.2014.Sh.D
100 0 _aSherif Adel Elkhodary
245 1 0 _aDetection, Quantitation and Viability State of Enterococcus Faecalis During Endodontic Treatment as Determined by Using Sensitive Molecular Techniques :
_bIn vivo Study /
_cSherif Adel Elkhodary ; Supervised Jealan Mohamed Eshafei , Somia Abdelatif Eisa , Geraldine Mohamed Nasr
246 1 5 _aاكتشاف وتقدير كمي وحالة القابلية للحياة لإلنتيروكوكس فيكالس أثناء المعالجة الجذرية والذي يتحدد باستخدام طرق حساسية الجزئيات:
_b(دراسة حية)
260 _aCairo :
_bSherif Adel Elkhodary ,
_c2014
300 _a128 Leaves :
_bcharts , facsimiles ;
_c30cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Endodontics
520 _a The aim of the present in vivo study was the detection, quantitation and viability state of E. faecalis present in infected root canals before and after chemomechanical preparation using MTAD as an irrigant by culture and sensitive molecular techniques. A total of 50 patients of both sexes of age ranging from 18 to 40 years were enrolled. The patients required endodontic treatment for single rooted teeth. Patients were medically free and the teeth were necrotic asymptomatic with periapical lesions. Access cavities were made and samples were taken from the canals with paper points and transferred to the transport medium (S1 samples). S2 samples were taken from the canals after mechanical preparation and irrigation with 1.3% sodium hypochlorite and MTAD as a final rinse and then transferred to the transport medium. In the first part of this study, samples (S1 and S2) were taken to the microbiology lab for culture on CLED agar for detection and viability state of the bacteria. Subculturing was then done on EBA agar which is a selective media for E. faecalis. Gram stained film and catalase test were done for identification of the isolated bacteria
530 _aIssued also as CD
653 4 _aCLED agar
653 4 _aE. faecalis
653 4 _aMTAD
700 0 _aGeraldine Mohamed Nasr ,
_eSupervisor
700 0 _aJealan Mohamed Elshafei ,
_eSupervisor
700 0 _aSomia Abdelatif Eisa ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aAml
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c49817
_d49817