000 02958cam a2200349 a 4500
003 EG-GiCUC
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008 150728s2014 ua do f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.10.10.M.Sc.2014.Ma.I
100 0 _aMahmoud Tawfik Ahmed Ismail
245 1 0 _aInvestigation of enterotoxigenic and antimicrobial resistant staphylococci in imported frozen chicken /
_cMahmoud Tawfik Ahmed Ismail ; Supervised Mona Ibrahim Elenbaawy , Ahmed Samir , Soad Abdelaziz
246 1 5 _aالتحري عن وجود بكتيريا المكور العنقودي المسبب للتسمم المعوي ومقاومته للمضادات البكتيرية في الدواجن المجمدة المستوردة
260 _aCairo :
_bMahmoud Tawfik Ahmed Ismail ,
_c2014
300 _a144 P. :
_bcharts , photographs ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 _aA total of 200 imported frozen chicken samples were examined for enterotoxigenicity and antimicrobial sensitivity.80 sample of them were negative for staphylococci and 120 samples were positive for staphylococci. Integral system stafilococchi kit was used for identification of 6 isolates of coagulase positive staphylococci, which indicated S.aureus. And 14 isolates of coagulase negative, which indicated that; 50% were S.haemolyticus, 21% were S.cohnii, 14% were S.warneri, 7% were S.simulans, and 7% were S.capitis. By antimicrobial sensitivity tests for the 20 isolates; 80% of isolates were resistant to Gentamicin, 85% resistant to Tobramycin, 45% were resistant to Amoxicillin + clavulanic acid, 70% were resistant to Ceftazidime, 50% resistant to Cefuroxime,100% resistant to Azitromycin, 45% were resistant to Claritromycin, 60% were resistant to Erythromycin, 65% were resistant to Roxitromycin. Suckling mice assay was done for 10 isolates of coagulase negative staphylococci and 10 coagulase positive and showed that, all 10 coagulase positive were positive for enterotoxigenicity and all 10 coagulase negative were negative for enterotoxigenicity. The same 20 isolates were examined by PCR and showed that from coagulase positive; 2 isolates were positive for seb gene and 4 isolates were positive for sec. None of 10 coagulase negative staphylococci were positive for enterotoxigenic genes. In conclusion, Imported frozen chicken may represent public health hazard
530 _aIssued also as CD
653 4 _aCoagulase -ve staphylococci
653 4 _aCoagulase +ve staphylococci
653 4 _aStaphylococci
700 0 _aAhmed Samir ,
_eSupervisor
700 0 _aMona Ibrahim Elenbaawy ,
_eSupervisor
700 0 _aSoad Abdelaziz ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aEnas
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c51762
_d51762