| 000 | 02325cam a2200325 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 008 | 151129s2015 ua d f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.11.07.M.Sc.2015.Re.I | ||
| 100 | 0 | _aReem Hasanin Mohamed Ali | |
| 245 | 1 | 0 |
_aIncreased integrity of free circulating DNA in sera of patients with colorectal cancer / _cReem Hasanin Mohamed Ali ; Supervised Dina Farouk Elgyaar , Nevine Ezz Eldin Elabd , Noha Hassan Ibrahim |
| 246 | 1 | 5 | _aدراسة سلامة الحمض النووى الحر كعلامة بيولوجية مصلية محتملة للكشف عن سرطان القولون و المستقيم |
| 260 |
_aCairo : _bReem Hasanin Mohamed Ali , _c2015 |
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| 300 |
_a147 P. : _bcharts ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology | ||
| 520 | _aCell - free DNA circulating in blood is a candidate biomarker for malignant tumors. Unlike uniformly truncated DNA released from apoptotic non diseased cells, DNA released from dead cancer cells varies in size. To measure the ratio of longer to shorter DNA fragments (DNA integrity) in serum to study it as a potential biomarker for patients with colorectal cancer (CRC). Sera from 50 patients with CRC,10 patients with benign colonic lesions and 20 healthy volunteers were assessed by quantitative real-time PCR of ALU repeats (ALU-qPCR) with 2 sets of primers (115 and 247 bp) amplifying different lengths of DNA. DNA integrity was determined as ratio of qPCR results of ALU 247 - bp over ALU 115-bp. Serum DNA integrity was increased in cases of CRC at different stages indicating highly significant difference from control and premalignant groups (p < 0.001). ROC curves for differentiating CRC from control and from premalignant lesions had areas under the curves of 0.9 and 0.85 respectively | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aALU - qPCR | |
| 653 | 4 | _aColorectal cancer | |
| 653 | 4 | _aDNA integrity | |
| 700 | 0 |
_aDina Farouk Elgyaar , _eSupervisor |
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| 700 | 0 |
_aNevine Ezz Eldin Elabd , _eSupervisor |
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| 700 | 0 |
_aNoha Hassan Ibrahim , _eSupervisor |
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| 905 |
_aNazla _eRevisor |
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| 905 |
_aSamia _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c53602 _d53602 |
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