000			03003cam a2200349 a 4500
003			EG-GiCUC
005			20250223031347.0
008			151202s2015 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.25.M.Sc.2015.Na.R
100	0		_aNancy Mohamed Saad Elhossary
245	1	0	_aRegeneration of insulin- producing cells from wharton{u2019}s jelly mesenchymal stem cells for the treatment of type Ї diabetes mellitus / _cNancy Mohamed Saad Elhossary ; Supervised Amr Saad Elsayed , Hamdy Mahmoud Hassanein , Abdelwahab AbouBakr Elghareeb
246	1	5	_aتجدد الخلايا المىتجة للإنسولين من الخلايا الجذعية الوسيطة لهلام وارتون لعلاج مرض البول السكرى من النوع الاول
260			_aCairo : _bNancy Mohamed Saad Elhossary , _c2015
300			_a77 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry
520			_aRegenerative medicine represents one of the main concerns of medicine. The aim of this study is to investigate an alternative technique for islet transplantation to treat type Ї diabetes mellitus (T1DM) by efficiently inducing easily obtained and processed Wharton{u2019}s jelly {u2013} derived mesenchymal stem cells of human umbilical cord (huMSCs) to differentiate into islet-like cells (ILCs). HuMSCs were isolated, cultured and induced chemically by fibronectin to differentiate in vitro into ILCs). The response of huMSCs was observed under an inverted microscope and evaluated by flow cytometry, H&E staining and immunocytochemistry. Twenty five male albino rats were divided into five groups, one of which was used as a control. Four groups were injected with 30 mg/kg of streptozotocin (STZ) on 3 consecutive days to induce T1DM. 5{u00D7}10⁶ of the differentiated huMSCs into functional insulin-producing cells were transplanted into two STZ-induced diabetic groups via two various ways , one via the tail vein and the other was intraperitoneally .While, 5{u00D7}10⁶ of the undifferentiated huMSCs were transplanted into the third group via the tail vein. Random blood glucose level (RBGL) and body weight (B.wt) were monitored weekly. After eight weeks, all rats were sacrificed and the pancreatic tissue was harvested in order to examine the change of pancreatic islets{u2019} structure, area and diameter by histomorphologic and histomorphometric analysis.
530			_aIssued also as CD
653		4	_aDiabetes mellitus
653		4	_aHuMSCs
653		4	_aRegenerative medicine
700	0		_aAbdelwahab AbouBakr Elghareeb , _eSupervisor
700	0		_aAmr Saad Elsayed , _eSupervisor
700	0		_aHamdy Mahmoud Hassanein , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSoheir _eCataloger
942			_2ddc _cTH
999			_c53703 _d53703