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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.12.04.Ph.D.2015.Mo.S
100 0 _aMona Saad Eldin Ibrahim Elneklawi
245 1 0 _aStimulation and control of cellular functions by extremely low frequency electromagnetic waves /
_cMona Saad Eldin Ibrahim Elneklawi ; Supervised Fadel Mohamed Ali , Reem Hassan Elgebaly
246 1 5 _aالتحفيز والتحكم فى نشاط الخلايا بواسطة الموجات الكهرومغناطيسية ذات التردد شديد الإنخفاض
260 _aCairo :
_bMona Saad Eldin Ibrahim Elneklawi ,
_c2015
300 _a116 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biophysics
520 _aEffect of extremely low frequency positive electric pulse with different duty cycles on pseudomonas aeruginosa (ATCC: 27853) growth by constructed and implemented exposure device was investigated in this study. The exposure device was applied to give extremely low frequency in the range of 0.1 Hz up to 20 Hz. Additional option in the exposure device was to control the duty cycle of the pulse with variation from 10% up to 100%. Growth curves of pseudomonas aeruginosa were investigated before and after exposure to different frequencies (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 Hz) through measuring the optical density and cell count for confirmation of data. Exposures to resonance frequency in the whole ranges of duty cycles provided by the designed device were done. These studies were followed by DNA fragmentation technique, transmission electron microscope (TEM), antibiotic susceptibility tests, and dielectric measurements for exposed and unexposed microorganism. Results revealed inhibition effect by 48.56 % and 47.4% together with change in the DNA structural properties for samples exposed to 0.5 Hz and 0.7 Hz respectively. Moreover the data indicated important role of duty cycle on the inhibition mechanism. Furthermore, two different mechanisms of interaction between positive electric pulse and microorganism occurred; 0.5 Hz caused rupture in cell wall while 0.7 Hz caused denaturation of the inner consistent of the cell.
530 _aIssued also as CD
653 4 _aCellular functions
653 4 _aElectromagnetic waves
653 4 _aLow frequency
700 0 _aFadel Mohamed Ali ,
_eSupervisor
700 0 _aReem Hassan Elgebaly ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSoheir
_eCataloger
942 _2ddc
_cTH
999 _c53907
_d53907