000			02810cam a2200325 a 4500
003			EG-GiCUC
008			151226s2015 ua o f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.24.04.Ph.D.2015.In.B
100	0		_aInas Sayed Mostafa Sayed
245	1	0	_aBiostimulatory effect of low level laser on gene expression of growth factors and cytokines in rat{u2019}s gingiva / _cInas Sayed Mostafa Sayed ; Supervised Ali Mohamed Elhoseni Saafan , Tarek Abdelhameed Harhash , Mostapha Ahmed Abdelrahman
246	1	5	_aدراسة التأثيرالتحفيزى لليزر منخفض الطاقة على التمثيل الجينى لمستحثات النمو و السيتوكينز فى لثه فئران التجارب
260			_aCairo : _bInas Sayed Mostafa Sayed , _c2015
300			_a114 P. : _bphotographs ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Applications in Oral Surgery
520			_aThe effect of low - level laser therapy (LLLT) on wound healing has been evaluated in several studies; however, little is known about the molecular mechanisms that underlies the biostimulatory effect of this treatment modality. To evaluate the effect of LLLT on gene expression of vascular endothelial growth factor (VEGF), interleukin - 1beta (IL - 1 Ý) and interferon Þ (IFN - Þ) during healing of wounds created by surgical blade and by surgical laser in order to have a better image of their interactive role influenced by the laser irradiation. 40 male wister rats were randomly assigned into four groups (A, B, C and D). In groups A and C, an incision was made in the gingival and mucosal tissues using a surgical scalpel. A similar incision was made in groups B and D using 980 nm diode laser. Group A and B were subjected twice to low level 870 nm diode laser 3 h and 24 h after the incision while group C and D served as controls. 30 min after the second irradiation, the rats belonging to all groups were euthanized, and the wound area was excised. Quantitative reverse - transcriptasepolymerase chain reaction (qRT - PCR) was used to measure the gene expression of VEGF, IL - 1Ý and IFN - Þ. To verify the results of gene expressions of these growth factors and cytokines, ELISA was used to measure their protein expression in the wound area
530			_aIssued also as CD
653		4	_aCytokines
653		4	_aLaser biostimulation
653		4	_aLLLT
700	0		_aAli Mohamed Elhoseni Saafan , _eSupervisor
700	0		_aMostapha Ahmed Abdelrahman , _eSupervisor
700	0		_aTarek Abdelhameed Harhash , _eSupervisor
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c54108 _d54108