000			02961cam a2200325 a 4500
003			EG-GiCUC
008			160103s2015 ua h f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.10.13.M.Sc.2015.Ah.E
100	0		_aAhmed Nour Eldine Abdallah
245	1	0	_aEvaluation of low level laser - activated adipose derived stem cells for treatment of experimental chondral defects / _cAhmed Nour Eldine Abdallah ; Supervised Ashraf Aly Eldesoky Shamaa , Omar Mohammed Salah Eldin Eltookhy , Eman Mohammed Abdelmottaleb
246	1	5	_aتقييم الخلايا الجذعية المستخلصة من النسيج الدهنى و المحفزة بأشعة الليزر فى علاج عيوب الغضاريف المستحدثة تجريبياً
260			_aCairo : _bAhmed Nour Eldine Abdallah , _c2015
300			_a98 P. : _bfacsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Surgery, Anesthesiology - Radiology
520			_aRegenerative therapies offer attractive alternatives for the treatment of chondral defects. Adipose - derived mesenchymal stem cells (AD - MSCs) found in the stromal vascular fraction (SVF) allow the development of one-step surgical procedure by their abundant availability and a minimal invasive technique. Low level laser irradiation (LLLI) of stem cells showed improved results on stem cell growth and proliferation. The aim of this work was to evaluate the treatment of chondral defects using single injection of laser activated SVF in a platelet rich plasma (PRP) vehicle. Chondral defects were created surgically in the femoral condoyle of 9 dogs. Autologous adipose tissue was harvested from the abdomen and SVF was isolated, added to the PRP, laser irradiated and injected intraarticularly in the right joint. The left joint was injected normal saline as control negative. Evaluation of the treatment included physical, radiological and histopathological examination. Treated joints showed marked degree of cartilage regeneration and restoration of the chondral histomorphological picture on the contrary to the control ones that showed deterioration overtime and defect filling with fibrous tissue forming a fibrocartilage at the end of 6 months period. The obtained results proved that the use of low level laser activated adipose derived stem cells is a safe, feasible technique as a single step surgical procedure and a very promising option for treatment of chondral defects
530			_aIssued also as CD
653		4	_aAdipose
653		4	_aStem cells
653		4	_aStromal vascular fraction
700	0		_aAshraf Aly Eldesoky Shamaa , _eSupervisor
700	0		_aEman Mohammed Abdelmottaleb , _eSupervisor
700	0		_aOmar Mohammed Salah Eldin Eltookhy , _eSupervisor
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c54241 _d54241