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008 160214s2015 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.07.06.M.Sc.2015.Ab.Y
100 0 _aAbdoulrhman Amir Abdou
245 1 0 _aYeasts as a promising tool for microbial oil production /
_cAbdoulrhman Amir Abdou ; Supervised Mohammed Zakaria Sedik , Ebtsam Mohammed Morsy
246 1 5 _aالخمائر كأداة واعدة لإنتاج الزيوت الميكروبية
260 _aCairo :
_bAbdoulrhman Amir Abdou ,
_c2015
300 _a135 P. :
_bcharts ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology
520 _aMicrobial lipid has high similarity to the oil obtained from plants and animals in type and composition. Production of oil from yeasts must be economical, so optimization of the cultivation conditions to produce higher production must be done. Lipomyces starkeyi was used for lipid production as a standard strain. Native oleaginous yeast, Rhodosporidium toruloides was isolated from soil and screened. This thesis studied effect of cell disruption methods and different types of solvent systems for lipid extraction. Several pre-production medium and production medium were studied for optimizing lipid production Also, the best carbon and nitrogen source was studied for lipid production. The research was conducted to design process optimization for condition factors through robust Taguchi design to identify the best parameters setting for lipid production. Analyzing of Taguchi data obtained the best level for each factor, that allow to form new NLM for each strain led to increase lipid contents reached 70.08 and 55.42% for R. toruloides and L. starkeyi, respectively. GC-MS was used for identifying and confirming the production of microbial oil in this study. Palmitic acid and oleic acid were major compounds for both strains. So, using R. toruloides in the economic production of lipids is recommended, and applies Taguchi design for more optimization processes
530 _aIssued also as CD
653 4 _aMicrobial oil
653 4 _aNitrogen- limited medium
653 4 _aOleaginous yeasts
700 0 _aEbtsam Mohammed Morsy ,
_eSupervisor
700 0 _aMohammed Zakaria Sedik ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aEnas
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c54967
_d54967