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008 160222s2015 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.10.03.Ph.D.2015.Em.F
100 0 _aEman Anter Morsy Ibrahim
245 1 0 _aFurther studies on bacterial diseases causing high mortality in broiler chicks /
_cEman Anter Morsy Ibrahim ; Supervised Diaa Eldein Gad Ahmed Khelfa , Wafaa Abdelghany Abdelghany
246 1 5 _aدراسات اخرى عن الامراض البكتيرية المسببة لارتفاع النفوق فى كتاكيت بدارى التسمين
260 _aCairo :
_bEman Anter Morsy Ibrahim ,
_c2015
300 _a305 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Animal Diseases
520 _aA monitoring study was carried out on one hundred and two broiler flocks located in nine Egyptian governorates during the period of 2012 - 2014 in a trial for isolation of responsible aerobic bacterial agents causing high chicks mortality in such flocks. The highest mortality percent was recorded in Cobb breed on the 12th day of age in Beni-Suef while the lowest mortality percent recorded in Sasso breed on the 25th day of age in Sharkia. Bacteriological investigation revealed that the Escherichia coli isolates were the predominant organism (42.9%) followed by Salmonella (21.7%) Klebsiella pneumonia(10.1%), Proteus mirabilis(6.2%) and Pseudomonas aeruginosa (4.5%). Serological identification of E. coli isolates revealed that both O158 and O78 serogroups were the most predominant isolates (18.6%) each, followed by serogroups O27(10.8%), O26 (6.8%), O18 and O55(3.9%), each, O6, O111 and O159 (2.9%), each, finally serogroup O1, O8, O15, O44, O114, O119, O142, O153, O166 and O169 (1.5%), each while un-typable strains were (13.7%). Salmonella serological identification revealed that Salmonella Infantis (SI) was the most predominant isolates (27.2%) followed by Salmonella Virchow (SV) (23.4%), Salmonella Enteritidis (SE)( 20.8%), Salmonella Gallinarum (SG) (14.3%), Salmonella Kentucky (SK) (10.4%) and Salmonella Typhimurium (ST) (3.9%). In vitro testing of the phenotypic properties of the 204 E. coli isolates, it was found that all tested isolates were Congo red positive. Conventional and a real-time PCR assay were used for the detection of rfbS, flijB, fliC, stmm and sefA genes of SG, SI, SK, ST and SE respectively where they sequenced and submitted on gene bank with accession numbers KP730600, KP760484, KP760485, KP763723 and KP793717
530 _aIssued also as CD
653 4 _aBroiler chicks
653 4 _aE. coli
653 4 _aSalmonella
700 0 _aDiaa Eldein Gad Ahmed Khelfa ,
_eSupervisor
700 0 _aWafaa Abdelghany Abdelghany ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aEnas
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c55126
_d55126