000			02886cam a2200349 a 4500
003			EG-GiCUC
005			20250223031434.0
008			160302s2015 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.21.M.Sc.2015.He.E
100	0		_aHebatalla Abdelraouf Mohamed
245	1	0	_aExpression and mutation frequency of some immunoregulatory genes in mice infected with certain Aspergillus fungal species / _cHebatalla Abdelraouf Mohamed ; Supervised Mohamed Akmal Elghor , Tahany Mohamed Ali Abdelrahman , Jehane Ibrahim Eid
246	1	5	_aالتعبير الجينى و معدل الطفرات فى بعض الجينات المنظمة للمناعة فى فئران المعمل المصابة بأنواع معينه من فطر الأسبرجيلس
260			_aCairo : _bHebatalla Abdelraouf Mohamed , _c2015
300			_a101 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc) - Cairo University - Faculty of Science - Department of Zoology
520			_aInvasive pulmonary aspergillosis (IPA) is a lethal complication of persistent neutropenia. In the pathogenesis of IPA, proinflammatory cytokines seem to play a key role. However, little progress has been made in comparing the production of pulmonary cytokines in mice with IPA caused by A. fumigatus and A. flavus at the molecular level. Therefore, the aim was to investigate the mutational and expression pattern of proinflammatory cytokine encoding genes in the lung of immunocompetent and immunocompromised mice infected with either species. The levels of mutational events and transcription of IL-1Ý and IL- 6 encoding genes in the pulmonary tissues of infected mice (with or without immunosuppression) were compared by means of semi-quantitative polymerase chain reaction and PCR-SSCP. Pulmonary fungal burden and histopathological changes were also assessed. The degree of DNA degredation and amounts of pulmonary IL-1Ý and IL-6 induced by A. fumigatus were much higher than those produced by A. flavus. The differential expressions of cytokines by two species were not associated with any mutational events within the selected sequences of the cytokines encoding genes. Our data may explain why A. fumigatus, but less commonly A. flavus, cause IPA and also confirm the contribution of DNA degradation pattern associated with severe inflammation to the pathogenesis of IPA
530			_aIssued also as CD
653		4	_aA. flavus
653		4	_aA. fumigatus
653		4	_aInvasive pulmonary aspergillosis
700	0		_aJehane Ibrahim Eid , _eSupervisor
700	0		_aMohamed Akmal Elghor , _eSupervisor
700	0		_aTahany Mohamed Ali Abderahman , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAml _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c55289 _d55289