000			02178cam a2200325 a 4500
003			EG-GiCUC
008			160305s2015 ua h f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.21.M.Sc.2015.Re.I
100	0		_aRehab Hamdy Mahmoud Mabrouk
245	1	0	_aIsolation and differentiation of myoblasts from human umbilical cord stem cells and muscles / _cRehab Hamdy Mahmoud Mabrouk ; Supervised Mahmoud Ahmed Amer , Abeer Ahmed Bahnasy , Mervat Mohamed fouad Eldeftar
246	1	5	_aعزل وتميز الخلايا المخلة للعضلات من الخلايا الجزعية فى الحبل السرى ومن العضلات فى الانسان
260			_aCairo : _bRehab Hamdy Mahmoud Mabrouk , _c2015
300			_a104 P. : _bfacsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520			_aThis study was carried out to compare myogenic differentiation of skeletal muscle explants and cord blood stem cells. I- In this study, 15 samples of umbilical cord blood were obtained, isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in vitro, the myogenic differentiation medium was added. The cells showed a change in their morphology myotube appeared. II- Twenty five samples of skeletal muscle (1x1 cm3) were obtained and minced using micro- explant technique .In vitro expansion was performed. Muscle precursor cells reached (75%-80%) confluence; cells were sub-cultured till myotube formation. III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of Desmin and Myo D genes were assessed using RT-PCR technique.
530			_aIssued also as CD
653		4	_aCells and Muscles
653		4	_aHuman Umbilical
653		4	_aMyoblasts
700	0		_aAbeer Ahmed Bahnasy , _eSupervisor
700	0		_aMahmoud Ahmed Amer , _eSupervisor
700	0		_aMervat Mohamed fouad Eldeftar , _eSupervisor
905			_aNazla _eRevisor
905			_aSoheir _eCataloger
942			_2ddc _cTH
999			_c55330 _d55330