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003 EG-GiCUC
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008 160405s2015 ua h f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.16.03.Ph.D.2015.Sa.S
100 0 _aSayed Ahmed Abdelrahman Elsayeh
245 1 0 _aStudies on the genetic improvement of Jatropha curcas in Egypt and South Africa /
_cSayed Ahmed Abdelrahman Elsayeh ; Supervised Atef Mohamed Zakaria Sarhan , Amira Shawky Soliman , Ahmed Osman Hamada
246 1 5 _aدراسات علي التحسين الوراثي في الجتروفا في مصر وجنوب أفريقيا
260 _aCairo :
_bSayed Ahmed Abdelrahman Elsayeh ,
_c2015
300 _a249 P. :
_bfacsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Institute of African Research and Studies - Department of Natural Resources
520 _aThis study was performed at the Natural Resources Department, Institute of African Research and Studies, Cairo University, Giza, Egypt. The experiments were done in Horticulture Research Institute, Agriculture Research Center, Fruits Breeding Department - Giza, Egypt during the period 2012 to 2014. The aim of this work was to optimize the culturing conditions for the micropropagation of J. curcas L. by using single node and leaf explants and to study the salinity tolerance by using mutation through tissue culture technique. The highest percentage of survival explants were achieved by using NaOCl at 20%. The highest percentage of survival explants were achieved by using 0.1 g/l MC for 15 minutes. The single node explants cultured on medium supplemented with 2,4-D at 4.0 mg/l recorded the highest callus formation percentage 3.0%. The callus formation percentage range between 0.3 {u2013} 1.7% according to different concentrations of 2,4-D from leaves. By using NAA at 4.0 mg/l gave high percentage of callus formation 2.7%; while, NAA at 1.0 mg/l showed low percentage of callus formation of single nodes. By using NAA at 2.0 and 4.0 mg/l resulted in high percentage of callus formation of leaves. The highest percentage of callus formation 4.0% was recorded with 2,4-D at 1.0 mg/l among NAA, IBA and IAA at 1.0 mg/l of single nodes. The highest percentage of callus formation 3.7, 3.2, 3.8 respectively was recorded with 2,4-D at 1.0 mg/l among different concentration of NAA, IBA and IAA of leaves. BA at 3.0 mg/l with IAA at 3.0 mg/l increased the shoot formation percentage from single node and leaf explants. Using kin at 2.0 mg/l + IBA at 0.2 mg/l was recorded the highest shoot formation percentage from single node and leaf explants. The control treatment was recorded no results
530 _aIssued also as CD
653 4 _aCallus formation
653 4 _aJ. curcas L.
653 4 _aPlant growth regulators
700 0 _aAhmed Osman Hamada ,
_eSupervisor
700 0 _aAmira Shawky Soliman ,
_eSupervisor
700 0 _aAtef Mohamed Zakaria Sarhan ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSoheir
_eCataloger
942 _2ddc
_cTH
999 _c55844
_d55844