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008 160406s2015 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.12.05.M.Sc.2015.Em.V
100 0 _aEman Mostafa Mostafa Aborawash
245 1 0 _aVirulence factors and antibiotic resistance of endogenous bacterial isolates from intraocular lens in individuals submitted to cataract surgery /
_cEman Mostafa Mostafa Aborawash ; Supervised Zeinat Kamel Mohamed , Maha Mohssen Abdelfattah
246 1 5 _aعوامل الضراوة ومقاومة المضادات الحيوية للعزلات البكتيرية من عدسة العين لمرضى جراحة المياه البيضاء
260 _aCairo :
_bEman Mostafa Mostafa Aborawash ,
_c2015
300 _a124 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany Microbiology
520 _aThe objective of this study was to identify the conjunctival microbiota of patients undergoing cataract surgery and evaluate its antibiotic resistance pattern. Also to in-vitro evaluate the antimicrobial effects of some essential oils against multidrug resistant isolates. Ocular samples were collected using swabs and polymethyl-metaacrylate (PMMA) intraocular lenses from seventy patients submitted to cataract surgery. Isolation and identification of bacteria was performed using conventional microbiological methods and API system. The sensitivity of 16 essential oils against isolated bacteria was tested using well diffusion method. Combination effect of essential oils and antibiotic was also examined. Thirty six strains were isolated from patients submitted to cataract surgery. Gram positive microorganisms represent 75% of the isolates with coagulase- negative Staphylococci (CoNS) the most common isolate (47.2%) followed by S.aureus (19.4%), whereas gram negative occurred in 25% of cases, with Moraxella spp. the most frequent Gram negative isolate. Antibiotic sensitivity test revealed that the highest antibiotic resistance was exhibited against ceftazidime (100%) followed by clindamycin (86%), ampicillin (80.5%), erythromycin (72.2%) and tetracycline (69.4%). Gentamycin displayed the best activity (55.5%) followed by chloramphenicol (44.4%) and vancomycin (38.88%)
530 _aIssued also as CD
653 4 _aCataract surgery
653 4 _aEndogenous bacterial isolates
653 4 _aIntraocular lens
700 0 _aMaha Mohssen Abdelfattah ,
_eSupervisor
700 0 _aZeinat Kamel Mohamed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSoheir
_eCataloger
942 _2ddc
_cTH
999 _c55876
_d55876