| 000 | 02871cam a2200325 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 008 | 160416s2015 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.08.08.Ph.D.2015.Ba.P | ||
| 100 | 0 | _aBahgat Mohammed Ezzat Bahgat Fayed | |
| 245 | 1 | 0 |
_aProduction and formulation of novel antibiotics from streptomyces / _cBahgat Mohammed Ezzat Bahgat Fayed ; Supervised Omaima Naim Elgazayerly , Magdy Ali Amin , Amal Mohamed Abdelgawad Hashem |
| 246 | 1 | 5 | _aإنتاج و صياغة مضادات حيوية جديدة منتجة بطرق مبتكرة من بكتريا الأستربتوميسيس |
| 260 |
_aCairo : _bBahgat Mohammed Ezzat Bahgat Fayed , _c2015 |
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| 300 |
_a208 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmaceutics | ||
| 520 | _aHere we explore the use of multiple orthologous integrating plasmid systems, based on the int/attP loci from phages TG1, SV1 and zBT1, to express the PKS for erythromycin in a heterologous Streptomyces host. Streptomyces strains containing the three polyketide synthase genes, eryAI, eryAII and eryAIII expressed from three different integrated plasmids produced the aglycone intermediate, 6-deoxyerythronolide B. A further pair of integrating plasmids, both derived from the zC31 int/attP locus, were constructed encoding a gene cassette for glycosylation of the aglycone intermediates, with and without the tailoring gene, eryF, required for the synthesis of erythronolide B. Liquid chromatography {u2013} mass spectrometry of the metabolites indicated the production of angolosaminyl-6dEB and angolosaminyl-EB. Erythromycin was formulated into PLGA nanoparticles. Entrapment efficiency of erythromycin in PLGA nanoparticles was determined microbiologically. The effect of PLGA amount, PLGA type, organic solvent, sonication probe diameter, sonication time, organic phase: aqueous phase ratio as well as effect of PVA percent on microbiologically determined entrapment efficiency was assessed. The results showed that microbiologically determined entrapment efficiency was significantly enhanced after optimizing different parameters to reach 51.1±0.68 % for the optimum formula (F10). The size of the nanoparticles produced by optimal formulation observed from SEM micrographs was around 100 nm with narrow particle size distribution | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aErythromycin | |
| 653 | 4 | _aIntegrating plasmids | |
| 653 | 4 | _aNanoparticles | |
| 700 | 0 |
_aAmal Mohamed Abdelgawad Hashem , _eSupervisor |
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| 700 | 0 |
_aMagdy Ali Amin , _eSupervisor |
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| 700 | 0 |
_aOmaima Naim Elgazayerly , _eSupervisor |
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| 905 |
_aNazla _eRevisor |
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| 905 |
_aSoheir _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c56032 _d56032 |
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