000			03013cam a2200325 a 4500
003			EG-GiCUC
008			160417s2015 ua o f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.10.10.M.Sc.2015.Al.P
100	0		_aAlaa Tharwat Abdelmonem
245	1	0	_aPCR gene detection among HARMONY collection of staphylococcus aureus from bovine milk and human nasal carriage epidemiological and genetic findings / _cAlaa Tharwat Abdelmonem ; Supervised Wagih Armanious Gad Elsaid , Sabry Darwish Morgan , Rasha Mohammed Mahmoud Khairy
246	1	5	_aاختبار البلمره المتسلسل للكشف عن جينات فى تجميع متوافق للميكروب العنقودى الذهبى من البان الابقار و حاملى الميكروب فى الانف فى الانسان نتائج و بائيه و جينيه
260			_aCairo : _bAlaa Tharwat Abdelmonem , _c2015
300			_a186 P. : _bphotographs ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520			_aStaphylococcus aureus (S.aureus) is considered one of the most important pathogens to humans and animals. The emergence of methicillin-resistant S. aureus (MRSA) strains and other antimicrobial agents has become a major concern .316 samples (216 from bovine milk and 100 from nasal swabs of human) from Minia governorate were used in this study for isolation and identification of S.aureus from milk in healthy and diseased animals as well as from human nasal carriers, detection of the diversity between animal and human isolates by using phenotypic methods and amplification of certain genes; coa and mec A using PCR. all S. aureus isolates of the study (45) showed expression of mec A gene, although 96.77% of bovine isolates only was cefoxitin resistant by disk diffusion method so the detection of mec A gene is important for determination of MRSA. The 30 isolates of bovine milk showed 7 types of coa gene. The sizes of PCR amplicons obtained ranged from approximately 80 to approximately 640 bps. On the other hand the 14 isolates of human nasal swabs showed 6 types of coa gene, the size of PCR amplicons obtained after amplification of isolates from human nasal swabs ranged from approximately 80 to approximately 800 bps.it was noted that one isolate from bovine milk, identified as coagulase positive by tube coagulase test was found to be negative with PCR amplification of the gene, so the use of both phenotypic and molecular detection of S. aureus strains is very important
530			_aIssued also as CD
653		4	_aAntimicrobial sensitivity
653		4	_aMRSA
653		4	_aS. aureus
700	0		_aRasha Mohammed Mahmoud Khairy , _eSupervisor
700	0		_aSabry Darwish Morgan , _eSupervisor
700	0		_aWagih Armanious Gad Elsaid , _eSupervisor
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c56048 _d56048