000			03017cam a2200325 a 4500
003			EG-GiCUC
008			160730s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.08.01.Ph.D.2016.He.C
100	0		_aHebatullah Samy Mohamed Helmy
245	1	0	_aComparative study of the effect of certain antitumor drugs on neoplastic transformations of rats liver / _cHebatullah Samy Mohamed Helmy ; Supervised Tarek Mohamed Kamal Motawi , Noha Ahmed Elboghdady , Abeer Moustafa Elsayed
246	1	5	_aدراسة مقارنة لتأثير بعض الأدوية المضادة للسرطان علي التحول السرطاني في كبد الجرذان
260			_aCairo : _bHebatullah Samy Mohamed Helmy , _c2016
300			_a122 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Biochemistry
520			_aCancer stem cells (CSCs) in hepatocellular carcinoma (HCC) possess tumor initiating, metastatic and drug resistance properties. This study was conducted to evaluate the effect of pegylated interferon-Ü2a (PEG-IFN-Ü2a) and 5-fluorouracil (5-FU) on the expression of CSCs markers and on specific pathways that contribute to the propagation of CSCs in HCC. HCC was initiated in rats using a single intraperitoneal dose of diethylnitrosamine (DENA) (200 mg/kg) and promoted by weekly subcutaneous injections of carbon tetrachloride (CCl4) for six weeks. After the appearance of dysplastic nodules, the animals received either PEG-IFN-Ü2a or 5-FU for eight weeks. CSCs markers (OV6, CD90) and molecules related to transforming growth factor-Ý (TGF-Ý) and other signaling pathways were assessed in hepatic tissues. PEG-IFN-Ü2a treatment effectively suppressed the hepatic expression of OV6 and CD90, ameliorated the diminished hepatic expression of TGF-Ý receptor II (TGF-ÝRII) and Ý2 spectrin (Ý2SP) and significantly reduced the elevated hepatic expression of TGF-Ý1, interleukin6 (IL6), signal transducer and activator of transcription3 (STAT3) and vascular endothelial growth factor (VEGF). In contrast, 5-FU treatment failed to reduce the over-expression of CSCs markers and barely affected the disrupted TGF-Ý signaling. Furthermore, it had no effect on angiogenesis or nitrosative stress. In conclusion, PEG-IFN-Ü2a, but not 5-FU, could reduce the propagation of CSCs during the progression of HCC by upregulating the disrupted TGF-Ý signaling, suppressing the IL6/STAT3 pathway and reducing angiogenesis
530			_aIssued also as CD
653		4	_a5-fluorouracil
653		4	_aHepatocellular carcinoma
653		4	_aPegylated interferon-Ü2a
700	0		_aAbeer Moustafa Elsayed , _eSupervisor
700	0		_aNoha Ahmed Elboghdady , _eSupervisor
700	0		_aTarek Mohamed Kamal Motawi , _eSupervisor
905			_aEnas _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c57265 _d57265