000			02736cam a2200325 a 4500
003			EG-GiCUC
008			160926s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.11.Ph.D.2016.Al.S
100	0		_aAlzahraa Muhammad Abdelrasoul Abdelatty
245	1	0	_aShort term dietary modifications alter mammary lipogenic gene expression in mid lactating dairy cow using novel non-invasive rna extraction technique / _cAlzahraa Muhammad Abdelrasoul Abdelatty ; Supervised Fathy Farouk Mohamed , Richard Arthur Erdman , Mohamed Ahmed Tony
246	1	5	_aدراسة تأثير التغيير في التغذية قصير المدي علي تعبير جينات دهن اللبن بإستخلاص المادة الوراثية بتقنية حديثة
260			_aCairo : _bAlzahraa Muhammad Abdelrasoul Abdelatty , _c2016
300			_a125 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Nutrition
520			_aMilk fat is the most variable component of milk, it is easily changed by nutritional and environmental factors rendering it a central point of interest for the daily market and scientists. Dietary manipulation can have a profound influence on milk fat synthesis and its fatty acid profile. However, our basic understanding of how dietary manipulations regulates mammary lipogenesis is limited by the use of mammary biopsy to obtain representative tissue sample for gene expression analysis, as repeated biopsies over short time interval from the same animal is not possible. Therefore, the general objective of this dissertation was to measure the sequential changes in milk fat concentration, milk fatty acid profile and mammary lipogenic gene expression that occur during the transition from normal to dietary induced either elevated or depressed milk fat concentration, using new non-invasive alternative technique by extracting RNA from cytosolic crest trapped in large fat globules. Two experiments were conducted: 1) feed restriction that induce negative energy balance, body fat mobilization, and elevated milk fat concentration, 2) dietary induced milk fat depression by the use of dietary CLA that resulted in depression in milk fat concentration.
530			_aIssued also as CD
653		4	_aCLA
653		4	_aCytosolic crescent
653		4	_aDairy cow
700	0		_aFathy Farouk Mohamed , _eSupervisor
700	0		_aMohamed Ahmed Tony , _eSupervisor
700	0		_aRichard Arthur Erdman , _eSupervisor
905			_aNazla _eRevisor
905			_aSoheir _eCataloger
942			_2ddc _cTH
999			_c57859 _d57859