000			02933cam a2200325 a 4500
003			EG-GiCUC
008			161023s2016 ua h f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.11.26.M.Sc.2016.Sh.T
100	0		_aShaimaa Mohammed Abdelaal
245	1	0	_aTrial for determination of antigenic relationship between Schistosoma mansoni and its intermediate snail / Shaimaa Mohammed Abdelaal ; _cSupervised Jomana Abdelaziz Ahmed , Mousa Abdelgawad Mousa , Sahar Zayed Abdelmaogood
246	1	5	_aمحاولة تحديد علاقة المستضاد بين البلهارسيا المعوية والقوقع العائل الوسيط
260			_aCairo : _bShaimaa Mohammed Abdelaal , _c2016
300			_a105 P. : _bfacsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Parasitology
520			_aIntestinal schistosomiasis is a serious health disease, caused by Schistosomamansoni. Biomphalariaalexandrinasnail is the intermediate host of S. mansoniin Egypt. Antigenic relationship between S.mansoniand B.alexandrinasnail both infected and noninfected with S.mansoni wasstudied.S.mansonicrude antigen was prepared from adult stage ofS.mansonicollected from experimentally infected mice. Crude antigens from both infected and non infectedB.alexandrina snail were also prepared after dissecting each animal snail into two parts foot and visceral hump. These antigens were fractionated using Sodium dodecyl sulfate Poly acrylamide Gel Electrophoresis (SDS PAGE). Common bands were detected among adult worm antigen( AWA) and both infected and non infectedB. alexandrina visceral hump antigens at 22,30 and 58 KDa , while a common band among AWA antigen and both infected and non infected foot antigens was detected at 40 KDa. Specific mice hyper immune serum (HIS)was prepared against each prepared antigen that was used in western blot.WB revealed that HIS of AWA is reactive to infected snailmore thannon infectedsnail. HIS of AWA identifieda common band in non infected snails both foot and visceral hump at 70 -75 KDaand identified a common band at 45 KDa in AWA and both infected and non infectedvisceral hump snail antigens.HIS of B.alexandrina Infected foot detected a common band at 68KDa amongboth infected and non infected foot antigens and AWA. HIS of B. alexandrina non infected visceral hump detecteda common band at 35 KDa among both infected and non infected visceral snail antigens and AWA
530			_aIssued also as CD
653		4	_aAntigens
653		4	_aBiomphalariaalexandrina
653		4	_aSchistosomamansoni
700	0		_aAbdelaziz Ahmed , _eSupervisor
700	0		_aMousa Abdel Gawad Mousa , _eSupervisor
700	0		_aSahar Zayed Abdel Maogood , _eSupervisor
905			_aNazla _eRevisor
905			_aSamah _eCataloger
942			_2ddc _cTH
999			_c58310 _d58310