000			02729cam a2200325 a 4500
003			EG-GiCUC
008			161115s2016 ua f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.11.07.M.Sc.2016.Hu.V
100	0		_aHuda Ali Moustafa Karmani
245	1	0	_aValue of circulating GPC3 m RNA in detecting patient with hepatocellular carcinoma / _cHuda Ali Moustafa Karmani ; Supervised Nehad Ahmad Mosaad , Ashraf Omar Abdelaziz , Yassmin Taha Elshewey
246	1	5	_aدور الحامض الريبوزومى الرسولى للجليبكان 3 فى الكشف عن المرضى المصابين بسرطان الكب
260			_aCairo : _bHuda Ali Moustafa Karmani , _c2016
300			_a133 P. ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
520			_aAim: Is to test the clinical performance of GLYPICAN 3 gene expression as a new and specific diagnostic molecular marker in hepatocellular carcinoma by real time PCR and its protein level in serum by ELISA. The serum GPC3 protein and GPC 3 m-RNA level were measured in 30 patients with HCC and 25 patients with cirrhotic liver disease (CLD) and 25 normal healthy control by RT-PCR and by ELISA. The median values of GPC3 m-RNA by RT-PCR in patients with HCC and with CLD and in control group were 2.68ng/mL, 0.843ng/mL and 0.2ng/ml respectively. We found There was no statistically significant elevation between the HCC group as compared to the CLD groups with (p = 0.407), while both groups were significantly higher than control group (p = 0.001). The median values of serum GPC3 protein by ELISA in patients with HCC and with CLD and in control group were 2.9ng/mL, 2.4ng/ml and 2.1ng/mL, respectively. We found that there was no statistically significant difference between the HCC and the CLD groups with (p = 0.488), while both groups were significantly higher than control group (p = 0.004). The median values of serum AFP by ELISA in patients with HCC and with CLD and in control groups were 171.0 ng/mL,13ng/ml and 2.3ng/mL, respectively. We found that. There was statistically significant difference between the HCC and the CLD groups with (p=0.000). And both of them were statistcally higher than the control group p = 0.000
530			_aIssued also as CD
653		4	_aELISA
653		4	_aGlypican-3
653		4	_aHepatocellular carcinoma
700	0		_aAshraf Omar Abdelaziz , _eSupervisor
700	0		_aNehad Ahmad Mosaad , _eSupervisor
700	0		_aYassmin Taha Elshewey , _eSupervisor
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c58618 _d58618