000			02156cam a2200349 a 4500
003			EG-GiCUC
005			20250223031624.0
008			161210s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.07.09.Ph.D.2016.He.M
100	0		_aHeba Sayed Mohamad Mostafa
245	1	0	_aMutagenesis and protoplast fusion of arthrobacter sp. for improved glucose isomerase production / _cHeba Sayed Mohamad Mostafa ; Supervised Sobhy Mohamed Mohsen , Wael Ahmed Bazaraa , Nagwa Mohamed Elshimi
246	1	5	_aالتطفير و دمج البروتوبلاست لخلايا الأرثروباكتر لتحسين إنتاج الجلوكوز أيزوميريز
260			_aCairo : _bHeba Sayed Mohamad Mostafa , _c2016
300			_a133 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Food Science and Technology
520			_aIn attempts to construct superior glucose isomerase-producing strains, four strains were screened. Both Arthrobacter sp. and A. missouriensis were proved as the highest producers (16.8 and 15.6 U.ml-1, respectively). UV and Ethyl Methane Sulfonate (EMS) were used for mutagenesis. The mutant EMS 60-25 D generated from Arthrobacter sp. exhibited the highest GI production in this study (49.7 U.ml-1) with 3.2 folds improvement than its wild type. Protoplast fusion technology was successfully. The fusant (C 3-2) exhibited the highest GI synthesis (2.75 folds the wild type). The highest GI- producing fusant C 3-2 was used for the continuous production of HFCS. The maximal glucose conversion was 36% after 12h of isomerization at 60{u00B0}C
530			_aIssued also as CD
653		4	_aGlucose isomerase
653		4	_aMutation
653		4	_aProtoplast fusion
700	0		_aNagwa Mohamed Elshimi , _eSupervisor
700	0		_aSobhy Mohamed Mohsen , _eSupervisor
700	0		_aWael Ahmed Bazaraa , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShaima _eCataloger
942			_2ddc _cTH
999			_c58951 _d58951