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008 161213s2016 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.11.19.Ph.D.2016.Do.H
100 0 _aDoaa Mahdy Mohamed Mahmoud Elwakel
245 1 0 _aHuman metapneumovirus pediatric respiratory infections :
_bComparing direct immunofluorescence versus polymerase chain reaction /
_cDoaa Mahdy Mohamed Mahmoud Elwakel ; Supervised Eman Ahmed Elseidi , Reham Ali Dwedar , Hanaa Ibrahim Rady
246 1 5 _aعـدوى الجهــاز التنفـسى لــدى الأطـفــال المسببــة بفيـــروس المـيتانيمـو البشـرى:
_b مقارنـة بين اختبـار الوميـض المناعى المباشر و التفاعـــل المتسلســـل لخميـــرة البلـمــرة
260 _aCairo :
_bDoaa Mahdy Mohamed Mahmoud Elwakel ,
_c2016
300 _a136 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology
520 _aHuman metapneumovirus has been recognized as a common cause of respiratory infections particularly in infants and young children. The aim of the study is to determine the frequency of hMPV infections among children less than 2 years of age and to define its clinical features and seasonal pattern over a single respiratory season. Direct fluorescent antibody (DFA) assay was compared to RT-PCR for the detection of hMPV in respiratory samples. Fifty pediatric patients presenting with acute respiratory tract infection were enrolled in the study. Nasopharyngeal aspirate specimens were subjected to DFA assay and real-time PCR for the presence of hMPV. It was found that hMPV accounted for 4 cases (8%) of the studied patients and the infection rate was higher (20%) among the 12{u2013}24 months age group. Moreover, hMPV-positive cases were mostly detected in winter months. Their main clinical features were respiratory distress (100%) and cough (100%), with pneumonia (75%) and bronchiolitis (25%) being the main clinical diagnoses among the studied patients. By considering the real-time PCR assay as the gold standard test for diagnosing hMPV, the sensitivity, specificity, PPV, NPV and test efficiency of DFA for the detection of hMPV were 50%, 100%, 100%, 95.8% and 96%, respectively. Direct fluorescent antibody assay for hMPV was a highly specific and simple test but of low sensitivity
530 _aIssued also as CD
653 4 _aDirect immunofluorescence
653 4 _aHuman metapneumovirus
653 4 _aPolymerase chain reaction
700 0 _aEman Ahmed Elseidi ,
_eSupervisor
700 0 _aHanaa Ibrahim Rady ,
_eSupervisor
700 0 _aReham Ali Dwedar ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSoheir
_eCataloger
942 _2ddc
_cTH
999 _c58973
_d58973