| 000 | 03083cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223031625.0 | ||
| 008 | 161213s2016 ua dh f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.11.19.Ph.D.2016.Do.H | ||
| 100 | 0 | _aDoaa Mahdy Mohamed Mahmoud Elwakel | |
| 245 | 1 | 0 |
_aHuman metapneumovirus pediatric respiratory infections : _bComparing direct immunofluorescence versus polymerase chain reaction / _cDoaa Mahdy Mohamed Mahmoud Elwakel ; Supervised Eman Ahmed Elseidi , Reham Ali Dwedar , Hanaa Ibrahim Rady |
| 246 | 1 | 5 |
_aعـدوى الجهــاز التنفـسى لــدى الأطـفــال المسببــة بفيـــروس المـيتانيمـو البشـرى: _b مقارنـة بين اختبـار الوميـض المناعى المباشر و التفاعـــل المتسلســـل لخميـــرة البلـمــرة |
| 260 |
_aCairo : _bDoaa Mahdy Mohamed Mahmoud Elwakel , _c2016 |
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| 300 |
_a136 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology | ||
| 520 | _aHuman metapneumovirus has been recognized as a common cause of respiratory infections particularly in infants and young children. The aim of the study is to determine the frequency of hMPV infections among children less than 2 years of age and to define its clinical features and seasonal pattern over a single respiratory season. Direct fluorescent antibody (DFA) assay was compared to RT-PCR for the detection of hMPV in respiratory samples. Fifty pediatric patients presenting with acute respiratory tract infection were enrolled in the study. Nasopharyngeal aspirate specimens were subjected to DFA assay and real-time PCR for the presence of hMPV. It was found that hMPV accounted for 4 cases (8%) of the studied patients and the infection rate was higher (20%) among the 12{u2013}24 months age group. Moreover, hMPV-positive cases were mostly detected in winter months. Their main clinical features were respiratory distress (100%) and cough (100%), with pneumonia (75%) and bronchiolitis (25%) being the main clinical diagnoses among the studied patients. By considering the real-time PCR assay as the gold standard test for diagnosing hMPV, the sensitivity, specificity, PPV, NPV and test efficiency of DFA for the detection of hMPV were 50%, 100%, 100%, 95.8% and 96%, respectively. Direct fluorescent antibody assay for hMPV was a highly specific and simple test but of low sensitivity | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aDirect immunofluorescence | |
| 653 | 4 | _aHuman metapneumovirus | |
| 653 | 4 | _aPolymerase chain reaction | |
| 700 | 0 |
_aEman Ahmed Elseidi , _eSupervisor |
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| 700 | 0 |
_aHanaa Ibrahim Rady , _eSupervisor |
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| 700 | 0 |
_aReham Ali Dwedar , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
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| 905 |
_aSoheir _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c58973 _d58973 |
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