000			03122cam a2200349 a 4500
003			EG-GiCUC
005			20250223031625.0
008			161213s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.18.M.Sc.2016.Ra.B
100	0		_aRakan Ahmed Hussein
245	1	0	_aBiological control of bacterial wilt disease in tomato by some endophytic rhizobacteria / _cRakan Ahmed Hussein ; Supervised Tahany M. A. Abdelrahman , Gihan M. Z. Mounier , Mohamed Gamal Farahat
246	1	5	_aالسيطرة البيولوجية لمرض الذبول البكتيرى فى الطماطم بواسطة بعض انواع البكتريا الداخلية الجذرية
260			_aCairo : _bRakan Ahmed Hussein , _c2016
300			_a97, 21 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Microbiology
520			_aBacterial wilt disease caused by Ralstonia solanacearum is a serious threat for agricultural production of tomato. In this study, 80 endophytic bacterial isolates were screened from healthy tomato plants in R. solanacearum-infested fields. Two endophytic antagonists designated HRA32 and HRA69, showing the highest antagonistic activity via in vitro screening, were identified as Pseudomonas fluorescens and Bacillus subtilis using 16S rRNA analysis. The selected isolates were evaluated, in vitro, for their activities related to plant nutrition and plant growth regulation. Both of the assessed endophytes were found to exhibit capabilities in ammonia, indoleacetic acid (IAA) and siderophore production as well as phosphate solubilization. Pot experiments were adopted to assess the growth promoting activity and control efficiency of selected endophytes against tomato bacterial wilt. Results revealed that isolates HRA32 and HRA69 significantly enhanced all measured growth criteria and yield increased by 23.8% over untreated control. Clear synergetic effect was observed in tomato plants treated with a mixture of the two antagonists by reducing disease incidence significantly from 87.22% in the control to 16.66% with biological control efficacy of 80.23%. Both endophytes were considered as elicitors that induced elevated levels of phenolic compounds and defence enzymes including phenylalanine ammonia lyase, peroxidase and polyphenol oxidase. It is concluded that application of P. fluorescens HRA32 and B. subtilis HRA69 may be a promising approach for improving tomato yield and controlling bacterial wilt disease and may play an important role in sustainable agriculture.
530			_aIssued also as CD
653		4	_aBacterial wilt
653		4	_aEndophytic rhizobacteria
653		4	_aTomato
700	0		_aGihan Moner Zaghlol Mounier , _eSupervisor
700	0		_aMohamed Gamal Farahat , _eSupervisor
700	0		_aTahany Mohamed Abdelrahman , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSoheir _eCataloger
942			_2ddc _cTH
999			_c58980 _d58980