000			02867cam a2200337 a 4500
003			EG-GiCUC
005			20250223031634.0
008			170102s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.05.M.Sc.2016.Ha.M
100	0		_aHadeel Mosfer Dakhil Aljaeed
245	1	0	_aMolecular characterization between aflatoxinogenic and non- aflatoxinogenic strains of aspergillus flavus and aspergillus parasiticus / _cHadeel Mosfer Dakhil Aljaeed ; Supervised Mohamed Hani Moubasher , Amira Abu Taleb
246	1	5	_aالتوصيف الجزيئي بين السلالات المنتجة والسلالات الغير منتجة لسموم الافالتوكسين فطري الاسبرجيلاس فلافس والاسبيرجيلس بارازيتيكس
260			_aCairo : _bHadeel Mosfer Dakhil Aljaeed , _c2016
300			_a88 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology
520			_aNot all A. flavus and A. parasiticus are aflatoxigenic. - Eight strains were aflatoxigenic and three were non aflatoxigenic out of eleven strains. Five strains of aflatoxinogenic fungi were A. flavus var. columnaris, two strains were A. parasiticus, and one strain was A. flavus. Three isolates were n o n-aflatoxinogenic. Two of which were A. flavus var columnaris and one A. flavus. - All aflatoxin-producing strains could release both aflatoxin B and G groups - Aflatoxin production was affected by broth type as shown in A. flavus var. columnaris strains 9022, 9024, 9025, 9030, 9031 A. flavus strain 9027 and A. parasiticus strains 9029, 9032. - In spite of, TLC distinguished between aflatoxin-producing and non-producing strains, it failed in some cases to detect aflatoxin- producing strains because instability of aflatoxin production may occur in certain toxinogenic strains. - Multiplex RT-PCR for the selected genes also showed a good correlation between aflatoxin gene expression and aflatoxin production. - Expression of nor-1 (Afl-D), omt- B (Afl-O), omt-A ((Afl-P)) and ord-A (Afl-Q) genes provided useful differentiation of aflatoxigenic and non- aflatoxigenic strains of A. flavus and A. parasiticus. - The expression of the selected genes varied with regard to the aflatoxin- producing ability and types of media
530			_aIssued also as CD
653		4	_aAflatoxinogenic and non- aflatoxinogenic strains
653		4	_aAspergillus flavus and aspergillus parasiticus
653		4	_aMolecular characterization
700	0		_aAmira Abutaleb , _eSupervisor
700	0		_aMohamed Hani Moubasher , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aEnas _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c59294 _d59294